Cancer-associated fibroblasts enhance the migration ability of ovarian cancer cells by increasing EZH2 expression
Cancer-associated fibroblasts enhance the migration ability of ovarian cancer cells by increasing EZH2 expression
复制标题
癌症相关成纤维细胞通过增加 EZH2 表达来增强卵巢癌细胞的迁移能力。
DOI：
10.3892/ijmm.2013.1549
复制
发表时间：
2014-01-01
影响因子：
5.4
通讯作者：
Wang, Zehua
中科院分区：
文献类型：
--
作者：
Xu, Linjuan;Deng, Qingchun;Wang, Zehua
肿瘤微环境被认为影响恶性转化和肿瘤进展。组蛋白甲基转移酶，增强子zeste同源物2（EZH 2），最近被认为在几种类型的人类癌症的肿瘤发生中发挥关键作用。本研究旨在研究癌相关成纤维细胞（CAFs）对卵巢癌细胞EZH 2表达和迁移能力的影响，探讨肿瘤微环境与表观遗传调控之间的联系。将卵巢癌细胞系A2780、SKOV 3和ES 2与原代卵巢CAFs或正常成纤维细胞（NF）间接共培养。Transwell迁移实验检测卵巢癌细胞的迁移能力。通过定量逆转录PCR（qRT-PCR）和western blot分析来评估EZH 2的表达水平。将A2780-shEZH 2细胞（转染EZH 2靶向shRNA的A2780细胞）与CAFs或NF间接共培养，检测EZH 2表达水平的变化及细胞迁移能力。与CAFs共培养的A2780、SKOV 3和ES 2细胞的迁移能力明显增强（P
The tumor microenvironment is thought to affect malignant transformation and tumor progression. The histone methyltransferase, enhancer of zeste homologue 2 (EZH2), has recently been suggested to play a critical role in the tumorigenesis of several types of human cancer. The aim of this study was to investigate the effects of cancer-associated fibroblasts (CAFs) on the expression of EZH2 and the migration ability of ovarian cancer cells, in order to explore the link between the tumor microenvironment and epigenetic regulation. The ovarian cancer cell lines, A2780, SKOV3 and ES2, were indirectly co-cultured with primary ovarian CAFs or normal fibroblasts (NFs). The migration ability of the ovarian cancer cells was determined by Transwell migration assay. The expression levels of EZH2 were assessed by quantitative reverse transcription PCR (qRT-PCR) and western blot analysis. The A2780-shEZH2 cells (A2780 cells transfected with shRNA targeting EZH2) were indirectly co-cultured with CAFs or NFs, and the changes in the expression levels of EZH2 and the migration ability of the cells were detected. The migration ability of the A2780, SKOV3 and ES2 cells co-cultured with CAFs was significantly enhanced (P