Miniaturised Marine Algae Test with Polycyclic Aromatic Hydrocarbons - Comparing Equilibrium Passive Dosing and Nominal Spiking.
Miniaturised Marine Algae Test with Polycyclic Aromatic Hydrocarbons - Comparing Equilibrium Passive Dosing and Nominal Spiking.
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DOI：
10.1016/j.aquatox.2018.03.002
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发表时间：
2018-05
影响因子：
4.5
通讯作者：
N. C. Niehus;Carolin Floeter;H. Hollert;G. Witt
中科院分区：
文献类型：
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作者：
N. C. Niehus;Carolin Floeter;H. Hollert;G. Witt
在本研究中，基于ISO EN DIN10253开发了被动剂量的小型海藻试验（mMAT），以研究多环芳烃（PAHs）对海洋硅藻褐藻（phaeodactylum tricornutumum）生长的抑制作用。在水生毒性试验中，对多环芳烃等疏水有机化合物（hoc）的风险评估非常困难，因为它们的水溶性低、通过吸附到井中而损失以及挥发。然而，被动给药可以克服这些挑战。本研究采用生物相容性硅胶o型环作为多环芳烃储层。饱和状态下的单个多环芳烃使用被动剂量进行测试，并与标称峰值进行比较。此外，采用被动剂量法测试了多环芳烃的混合物，反映了沉积物孔隙水的现场组成。在被动给药试验中，多环芳烃显示出对藻类生长的强烈抑制作用，而在最高浓度下，名义峰值只有轻微的抑制作用。重建的多环芳烃混合物显示出轻微的抑制作用，使用被动剂量时，测试了5000倍的现场浓度。本研究证明了被动给药相对于峰值的优越性，并进一步成功地在海藻试验中实施了被动给药，使hoc的浓度保持在logKOW bbb4.6的恒定水平。
In this study the miniaturised Marine Algae Test (mMAT) using passive dosing was developed based on the ISO EN DIN10253 to investigate the growth inhibition of the marine diatomPhaeodactylum tricornutumcaused by polycyclic aromatic hydrocarbons (PAHs). Risk assessment of hydrophobic organic compounds (HOCs) like PAHs in aquatic toxicity tests is very difficult due to their low aqueous solubilities, losses via sorption to the wells and volatilisation. However, passive dosing can overcome these challenges. In this study biocompatible silicone O-rings were used as PAH reservoir. Individual PAHs at saturation were tested using passive dosing and in comparison with nominal spiking. Additionally, a recreated mixture of PAHs reflecting the field composition of the sediment pore water was tested with passive dosing. PAHs revealed strong growth inhibiting effects on algal growth in passive dosing tests, while nominal spiking had only slightly growth inhibiting effects in the highest concentration. The recreated PAH mixture revealed slightly inhibiting effects using passive dosing when tested with a factor of 5000 of the field concentration. This study demonstrates the superiority of passive dosing to spiking and further the successful implementation of passive dosing in the marine algae test maintaining a constant concentration for HOCs with a logKOW>4.6.