Nucleosome positioning in human HOX gene clusters
Nucleosome positioning in human HOX gene clusters
复制标题
DOI：
10.1101/gr.075952.107
复制
发表时间：
2008-10-01
期刊：
影响因子：
7
通讯作者：
Park, Peter J.
中科院分区：
文献类型：
--
作者：
Kharchenko, Peter V.;Woo, Caroline J.;Park, Peter J.
核小体沿着基因组的分布是染色质结构的重要方面，并且被认为通过调节DNA可及性来影响基因调控。然而，核小体组织的性质仍然知之甚少，特别是在哺乳动物基因组中。为了实现这一目标，我们使用平铺微阵列来确定人类细胞系中HOX基因簇沿着的稳定核小体位置。我们发现，核小体的位置表现出序列特性和长程组织，是不同于其他生物体的特点。尽管总体变异的核小体间距，特定的位点包含定期的核小体阵列与195 bp的周期性。此外，这种阵列倾向于优先发生在基因的3'端。通过比较不同的细胞系，我们发现，活跃的转录与增加定位的核小体，这表明了一个意想不到的作用，转录在建立良好定位的核小体。
The distribution of nucleosomes along the genome is a significant aspect of chromatin structure and is thought to influence gene regulation through modulation of DNA accessibility. However, properties of nucleosome organization remain poorly understood, particularly in mammalian genomes. Toward this goal we used tiled microarrays to identify stable nucleosome positions along the HOX gene clusters in human cell lines. We show that nucleosome positions exhibit sequence properties and long-range organization that are different from those characterized in other organisms. Despite overall variability of internucleosome distances, specific loci contain regular nucleosomal arrays with 195-bp periodicity. Moreover, such arrays tend to occur preferentially toward the 3' ends of genes. Through comparison of different cell lines, we find that active transcription is correlated with increased positioning of nucleosomes, suggesting an unexpected role for transcription in the establishment of well-positioned nucleosomes.