Reduced Responsiveness to Long-Term Monocular Deprivation of Parvalbumin Neurons Assessed by c-Fos Staining in Rat Visual Cortex
Reduced Responsiveness to Long-Term Monocular Deprivation of Parvalbumin Neurons Assessed by c-Fos Staining in Rat Visual Cortex
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DOI：
10.1371/journal.pone.0004342
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发表时间：
2009-02-04
期刊：
影响因子：
3.7
通讯作者：
Pizzorusso, Tommaso
中科院分区：
文献类型：
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作者：
Mainardi, Marco;Landi, Silvia;Pizzorusso, Tommaso
背景资料：一般认为，视觉皮层细胞均匀地改变其眼优势（OD），以响应单眼剥夺（MD），但很少有实验证据直接支持这一观点。通过使用免疫组化的活性依赖性标记c-Fos和弧，再加上抑制性皮质亚群的标记物染色，我们研究了是否长期MD在P21开始差异影响大鼠双眼初级视觉cortex.Methodology/Principal Findings抑制性神经元的视觉反应：GAD 67，parvalbumin（PV），calbindin（CB）和calretinin（CR）被使用。视觉激活Arc未与PV共定位，并从进一步研究中丢弃。MD可降低视觉诱导的GAD 67和CR阳性神经元c-Fos激活。CB群体对MD的反应是CB表达降低，而PV细胞对MD对c-Fos表达没有任何影响。在PV细胞中由剥夺性眼刺激诱导的c-Fos表达的持续性不太可能是由于活性依赖性c-Fos诱导的特别低的阈值。事实上，c-Fos诱导增加浓度的GABAA拮抗剂印防己毒素在视觉皮层切片PV细胞和其他皮质neurons.Conclusion之间是相似的：这些数据表明，PV细胞是特别难治性MD，这表明不同的皮质亚群可能会显示不同的反应MD。
Background: It is generally assumed that visual cortical cells homogeneously shift their ocular dominance (OD) in response to monocular deprivation (MD), however little experimental evidence directly supports this notion. By using immunohistochemistry for the activity-dependent markers c-Fos and Arc, coupled with staining for markers of inhibitory cortical sub-populations, we studied whether long-term MD initiated at P21 differentially affects visual response of inhibitory neurons in rat binocular primary visual cortex.Methodology/Principal Findings: The inhibitory markers GAD67, parvalbumin (PV), calbindin (CB) and calretinin (CR) were used. Visually activated Arc did not colocalize with PV and was discarded from further studies. MD decreased visually induced c-Fos activation in GAD67 and CR positive neurons. The CB population responded to MD with a decrease of CB expression, while PV cells did not show any effect of MD on c-Fos expression. The persistence of c-Fos expression induced by deprived eye stimulation in PV cells is not likely to be due to a particularly low threshold for activity-dependent c-Fos induction. Indeed, c-Fos induction by increasing concentrations of the GABAA antagonist picrotoxin in visual cortical slices was similar between PV cells and the other cortical neurons.Conclusion: These data indicate that PV cells are particularly refractory to MD, suggesting that different cortical subpopulation may show different response to MD.