Cyclic L-lactide synthesis from lignocellulose biomass by biorefining with complete inhibitor removal and highly simultaneous sugars assimilation
Cyclic L-lactide synthesis from lignocellulose biomass by biorefining with complete inhibitor removal and highly simultaneous sugars assimilation
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DOI：
10.1002/bit.28082
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发表时间：
2022-03-21
影响因子：
3.8
通讯作者：
Bao, Jie
中科院分区：
文献类型：
--
作者：
He, Niling;Jia, Jia;Bao, Jie
环手性丙交酯是聚合高相对分子质量聚乳酸的单体化学品。环状L-丙交酯的合成是由L-乳酸缩聚成低分子量预聚体，然后解聚成环状L-丙交酯。木质纤维素生物质是最有前途的生产乳酸的碳水化合物原料，但以木质纤维素生产的L乳酸为原料合成环状L丙交酯的研究尚未取得成功。主要障碍是粗纤维L-乳酸产品中残糖和抑制剂的杂质。在这里，我们成功地以纤维素L-乳酸为原料，通过木质纤维素生物精制，完全去除抑制剂和配位糖同化，合成了L-丙交酯。木质纤维素预处理中抑制剂的去除是通过使用一种独特的真菌树脂魔芋ZN1进行生物解毒完成的。工程菌L-乳酸链球菌能以相同的速率同时完全吸收非葡萄糖糖类。麦草发酵的L乳酸产量与玉米淀粉相当，光学纯度高(99.6%)，L乳酸效价高(129.4 g/L)，残留总糖少(接近2.2g/L)，且不含抑制剂。以规整提纯的L乳酸为原料，成功地合成了环状L丙交酯，并进行了详细的表征。本研究为木质纤维素生物质碳中性生产可生物降解聚乳酸奠定了技术基础。
Cyclic chiral lactide is the monomer chemical for polymerization of high molecular weight polylactic acid (PLA). The synthesis of cyclic L-lactide starts from polycondensation of L-lactic acid to a low molecular weight prepolymer and then depolymerized to cyclic L-lactide. Lignocellulose biomass is the most promising carbohydrate feedstock for lactic acid production, but the synthesis of cyclic L-lactide from L-lactic acid produced from lignocellulose has so far not been successful. The major barriers are the impurities of residual sugars and inhibitors in the crude cellulosic L-lactic acid product. Here we show a successful cyclic L-lactide synthesis from cellulosic L-lactic acid by lignocellulose biorefining with complete inhibitor removal and coordinated sugars assimilation. The removal of inhibitors from lignocellulose pretreatment was accomplished by biodetoxification using a unique fungus Amorphotheca resinae ZN1. The nonglucose sugars were completely and simultaneously assimilated at the same rate with glucose by the engineered L-lactic acid bacterium Pediococcus acidilactici. The L-lactic acid production from wheat straw was comparable to that from corn starch with high optical pure (99.6%), high L-lactic acid titer (129.4 g/L), minor residual total sugars (similar to 2.2 g/L), and inhibitors free. The cyclic L-lactide was successfully synthesized from the regularly purified L-lactic acid and verified by detailed characterizations. This study paves the technical foundation of carbon-neutral production of biodegradable PLA from lignocellulose biomass.