New water-compatible modified polystyrene as a stationary phase for high-performance liquid chromatography : Characterization and application
New water-compatible modified polystyrene as a stationary phase for high-performance liquid chromatography : Characterization and application
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DOI：
10.1016/s0021-9673(01)94524-8
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发表时间：
1987
影响因子：
4.1
通讯作者：
Y. Yang;M. Verzele
中科院分区：
文献类型：
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作者：
Y. Yang;M. Verzele
通过将中性亲水基团共价键合到聚苯乙烯-二乙烯基苯多孔珠表面，得到了一种新型的高效液相色谱填料。考察了平均粒径为7μm、平均孔径为3 nm和17 nm的颗粒作为固定相的刚性、稳定性、层析性能以及孔径对柱效率的影响等，改性聚苯乙烯保持了聚苯乙烯的主要优点：无离子，化学稳定性高。值得注意的还有刚性的增加，对溶剂从水到有机和从水到有机的兼容性，以及在不同的层析模式下的广泛应用范围。新型亲水性聚苯乙烯高效液相色谱相易于填充，柱寿命好。并举例说明了它在几种复杂混合物分离中的应用。
A new packing material for high-performance liquid chromatography (HPLC) was obtained by covalent bonding of neutral hydrophilic groups to the surface of porous polystyrene—divinylbenzene beads. Particles with a mean bead size of 7 μm and with mean pore diameters of 3 and 17 nm were evaluated as stationary phases as regards their rigidity, stability, chromatographic performance as well as the influence of pore size on column efficiency, etc. The modified polystyrene maintains the main advantages of polystyrene: it is ion-free and has high chemical stability. Noteworthy also are the increased rigidity, compatibility for solvent changes from aqueous to organic and the reverse and a broad application range in different chromatographic modes. The new hydrophilic polystyrene HPLC phase is easy to pack and yield good column lifetime. Its application to the separation of several complex mixtures is illustrated.