Impaired spatial learning in a novel rat model of mild cerebral concussion injury
Impaired spatial learning in a novel rat model of mild cerebral concussion injury
复制标题
DOI：
10.1016/j.expneurol.2005.06.013
复制
发表时间：
2005-10-01
影响因子：
5.3
通讯作者：
Schwab, S
中科院分区：
文献类型：
--
作者：
Henninger, N;Dützmann, S;Schwab, S
本研究旨在建立一种模拟人震荡性脑损伤的大鼠轻度创伤性脑损伤模型，为研究其病理生理学和潜在的治疗方法提供依据。34只雄性Wistar大鼠闭合性脑外伤（TBI）， 30只动物作为对照组（CON）。创伤后，动物立即失去肌肉张力和翻正反射反应，后者在11.4 +/- 8.2分钟内恢复。角膜反射和须反应分别在4.5 +/- 3.0 min和6.1 +/- 2.9 min内恢复。影响导致短暂性pO(2)降低（P < 0.001），平均动脉血压升高（P = 0.026），心率降低（P < 0.01）。在所有研究时间点，序列MRI未显示整个大脑弥散、T1、T2和T2*加权图像有任何异常。与对照组相比，TBI动物在Morris水迷宫中找到隐藏平台所需的时间明显更长，在训练象限中花费的时间也更少。创伤后第9天，脑外伤导致额叶皮质以及海马CA3 （P = 0.017）和CA1 （P = 0.002）显著的神经元损失（P < 0.001）；然而，通过正常β - APP-和MAP-2染色显示，细胞骨架结构得以保留。我们提出了一个独特的、无创的大鼠轻度闭合性头部创伤模型，具有人类脑震荡损伤的特征，包括短暂的意识丧失、认知障碍和轻微的脑损伤。(c) 2005爱思唯尔公司版权所有。
The aim of the present study was to develop a model of mild traumatic brain injury in the rat that mimics human concussive brain injury suitable to study pathophysiology and potential treatments. 34 male Wistar rats received a closed head trauma (TBI) and 30 animals served as controls (CON). Immediately following trauma, animals lost their muscle tone and righting reflex response, recovering from the latter within 11.4 +/- 8.2 min. Corneal reflex and whisker responses returned within 4.5 +/- 3.0 min and 6.1 +/- 2.9 min, respectively. The impact resulted in a short transient decrease of pO(2) (P < 0.001), increase in mean arterial blood pressure (P = 0.026), and a reduction of heart rate (P < 0.01). Serial MRI did not show any abnormalities across the entire cerebrum on diffusion, T1, T2, and T2*-weighted images at all investigated time points. TBI animals needed significantly longer to locate the hidden platform in a Morris water maze and spent less time in the training quadrant than controls. TBI led to a significant neuronal loss in frontal cortex (P < 0.001), as well as hippocampal CA3 (P = 0.017) and CA1 (P = 0.002) at 9 days after the trauma; however, cytoskeletal architecture was preserved as indicated by normal beta APP- and MAP-2 staining. We present a unique, noninvasive rat model of mild closed head trauma with characteristics of human concussion injury, including brief loss of consciousness, cognitive impairment, and minor brain injury. (c) 2005 Elsevier Inc. All rights reserved.