Antitumor activity and immune enhancement of murine interleukin-23 expressed in murine colon carcinoma cells.
Antitumor activity and immune enhancement of murine interleukin-23 expressed in murine colon carcinoma cells.
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发表时间：
2006-02
影响因子：
24.1
通讯作者：
B. Shan;Jing-sheng Hao;Qiao-xia Li;M. Tagawa
中科院分区：
文献类型：
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作者：
B. Shan;Jing-sheng Hao;Qiao-xia Li;M. Tagawa
白细胞介素 (IL)-23 是一种由 IL-12 的 p19 和 p40 亚基组成的细胞因子，可以增强记忆 T 细胞的增殖和活化 T 细胞产生 IFN-γ。它还可以在小鼠模型中诱导抗肿瘤作用。为了进一步评估 IL-23 的体内抗肿瘤活性和免疫增强作用，将逆转录病毒转导 mIL-23 基因的小鼠结肠癌细胞皮下注射到 BALB/c 小鼠体内。观察存活时间和肿瘤体积。 LDH释放测定、[3H]-TdR掺入测定和ELISA分别用于测定CTL活性、脾细胞增殖和细胞因子水平。通过流式细胞术（FCM）分析树突状细胞（DC）的数量。 Colon26/IL-23细胞分泌的IL-23抑制肿瘤生长，延长小鼠生存时间，增强脾细胞增殖、CTL活性和DC数量。 IL-23还促进Th1细胞因子的产生，例如IFN-γ、IL-12和TNF-α。然而，IL-4的水平并没有显着增强。这些数据表明肿瘤细胞分泌的IL-23可以通过增强免疫反应来诱导抗肿瘤活性。
Interleukin (IL)-23, a cytokine composed of p19 and the p40 subunit of IL-12, can enhance the proliferation of memory T cells and production of IFN-gamma from activated T cells. It can also induce antitumor effects in murine model. To further evaluate the antitumor activity and immune enhancement of IL-23 in vivo, murine colon carcinoma cells retrovirally transduced with mIL-23 gene were injected subcutaneously (s.c.) into BALB/c mice. Survival time and tumor volume were observed. LDH release assay, [3H]-TdR incorporation assay and ELISA were used to determine CTL activity, proliferation of splenocytes and level of cytokines, respectively. Number of dendritic cells (DCs) was analyzed by flow cytometry (FCM). IL-23 secreted by Colon26/IL-23 cells suppressed the growth of tumor and prolonged the survival time of mice, enhanced proliferation of splenocytes, CTL activity, and number of DCs. IL-23 also promoted the production of Th1 cytokines such as IFN-gamma, IL-12 and TNF-alpha. However, the level of IL-4 was not enhanced significantly. These data suggested that IL-23 secreted by tumor cells can induce antitumor activity by enhancing immune response.