Signaling through both type I DPP receptors is required for anterior-posterior patterning of the entire Drosophila wing.
Signaling through both type I DPP receptors is required for anterior-posterior patterning of the entire Drosophila wing.
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发表时间：
1997
期刊：
影响因子：
4.6
通讯作者：
M. Singer;A. Penton;V. Twombly;F. Hoffmann;W. Gelbart
中科院分区：
文献类型：
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作者：
M. Singer;A. Penton;V. Twombly;F. Hoffmann;W. Gelbart
成虫盘表达的TGF-β超家族成员DPP在一个狭窄的条纹细胞沿着的前-后室边界是必要的适当的增长和图案的果蝇附属物。我们研究DPP受体的功能，以了解这种本地化的DPP表达如何产生其全球性的影响后，附件的发展。萨克斯管（sax）或厚静脉（txm）突变细胞的克隆，在DPP的两种I型受体之一缺陷，显示细胞命运沿着前后轴的变化。在成人翼，纯合的空等位基因的萨克斯或亚纯型等位基因的taxi的克隆显示转移到更前的命运时，克隆是在前室和更后的命运时，克隆是在后室。这些克隆对下游基因Spalt-major的表达模式的影响也与细胞命运中的这些特定转变相关。sax无效和tkv亚型克隆的类似作用表明，这两种受体在翅型形成过程中功能的主要差异在于，TKV比SAX传递更多的DPP信号。我们的研究结果是一致的模型中，DPP的梯度达到所有细胞在发展中的翼片，以直接的前-后模式。
The imaginal disk expression of the TGF-beta superfamily member DPP in a narrow stripe of cells along the anterior-posterior compartment boundary is essential for proper growth and patterning of the Drosophila appendages. We examine DPP receptor function to understand how this localized DPP expression produces its global effects upon appendage development. Clones of saxophone (sax) or thick veins (tkv) mutant cells, defective in one of the two type I receptors for DPP, show shifts in cell fate along the anterior-posterior axis. In the adult wing, clones that are homozygous for a null allele of sax or a hypomorphic allele of tkv show shifts to more anterior fates when the clone is in the anterior compartment and to more posterior fates when the clone is in the posterior compartment. The effect of these clones upon the expression pattern of the downstream gene spalt-major also correlates with these specific shifts in cell fate. The similar effects of sax null and tkv hypomorphic clones indicate that the primary difference in the function of these two receptors during wing patterning is that TKV transmits more of the DPP signal than does SAX. Our results are consistent with a model in which a gradient of DPP reaches all cells in the developing wing blade to direct anterior-posterior pattern.