Beyond vasodilation: The antioxidant effect of adrenomedullin in Dahl salt-sensitive rat aorta
Beyond vasodilation: The antioxidant effect of adrenomedullin in Dahl salt-sensitive rat aorta
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DOI：
10.1016/j.bbrc.2005.05.034
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发表时间：
2005-07-08
影响因子：
3.1
通讯作者：
Kitamura, K
中科院分区：
文献类型：
--
作者：
Cao, YN;Kuwasako, K;Kitamura, K
我们研究了肾上腺髓质素（AM）对Dahl盐敏感（DS）大鼠高血压模型内皮功能的抗氧化作用。以耐盐大鼠（DR）和DS大鼠分别饲喂NaCl日粮。另外，给DS大鼠皮下注射生理盐水或重组人AM 4周。虽然AM和盐水灌注大鼠每周收缩压测量值无显著差异，但后者主动脉O-2中心点（-）水平显著升高（p0.01）。同样，在盐水输注的DS大鼠中，内皮型一氧化氮合酶（eNOS） mRNA和蛋白均显著升高，注入AM可将O-2（-）和eNOS的表达降低至与DR大鼠相当的水平。AM还上调了鸟苷-5′-三磷酸环水解酶I的基因表达，下调了p22（phox）的表达，表明AM增加了NOS的偶联性和NO的生物利用度。AM具有显著的抗氧化特性，可改善内皮功能，(c) 2005 Elsevier Inc.。版权所有。
We have investigated the antioxidant effect of adrenomedullin (AM) on endothelial function in the Dahl salt-sensitive (DS) rat hypertension model. Dahl salt-resistant (DR) and DS rats were fed an NaCl diet. In addition, the DS rats were subcutaneously infused with either saline or recombinant human AM for 4 weeks. Although systolic blood pressures measured weekly in AM- and saline-infused rats did not significantly differ, aortic O-2 center dot(-) levels were significantly (P 0.01) higher in the latter. Likewise, both endothelial nitric oxide synthase (eNOS) mRNA and protein were significantly higher in saline-infused DS rats, Infusion of AM reduced both O-2(-) and eNOS expression to levels comparable to those seen in DR rats. AM infusion also upregulated the gene expression of guanosine-5'-triphosphate cyclohydrolase I and downregulated the expression of p22(phox), suggesting that AM increased the NOS coupling and bioavailability of NO. AM possesses significant antioxidant properties that improve endothelial function, (c) 2005 Elsevier Inc. All rights reserved.