EXPERIMENTAL REPRODUCTION OF PORCINE EPIDEMIC ABORTION AND RESPIRATORY SYNDROME (MYSTERY SWINE DISEASE) BY INFECTION WITH LELYSTAD VIRUS - KOCH POSTULATES FULFILLED
EXPERIMENTAL REPRODUCTION OF PORCINE EPIDEMIC ABORTION AND RESPIRATORY SYNDROME (MYSTERY SWINE DISEASE) BY INFECTION WITH LELYSTAD VIRUS - KOCH POSTULATES FULFILLED
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DOI：
10.1080/01652176.1991.9694297
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发表时间：
1991-01-01
影响因子：
6.4
通讯作者：
POL, JMA
中科院分区：
文献类型：
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作者：
TERPSTRA, C;WENSVOORT, G;POL, JMA
8头怀孕母猪暴露于细胞培养繁殖的莱利斯塔病毒的气溶胶导致了所谓的神秘猪病的临床症状特征。 经过4-7天的孵化后，所有母猪都食欲不振，无精打采6-9天。 2头母猪出现短暂的耳红-蓝变色（“蓝耳病”），伴有腹式呼吸，2头母猪仅发热1天，1头母猪在妊娠109天流产。 其他7头母猪在妊娠113 - 117天之间分娩，产下许多木乃伊、死亡和虚弱的仔猪。 在这7头母猪中，每头母猪出生时死亡仔猪的平均数为4.6头，出生时存活仔猪的平均数为7.7头;每头母猪有3.1头仔猪（40%）在第一周内死亡。从31头出生时死亡或出生后不久死亡的仔猪中分离出莱利斯塔病毒。 在23头仔猪的前初乳血样或腹水中检测到抗体，这一发现表明8窝仔猪中有6窝经胎盘传播病毒。 我们得出结论，莱利斯塔病毒是神秘猪病的病原体。 由于其病因已不再是一个谜，我们建议更合适的名称“猪流行性流产和呼吸综合征（PEARS）”。
Aerosol exposure of eight pregnant sows to cell-culture-propagated Lelystad virus resulted in clinical signs characteristic of so-called mystery swine disease. After an incubation of 4-7 days, all sows were inappetant and listless for 6-9 days. Two sows developed a transient red-blue discolouration of the ears ('abortus blauw' or blue ear disease) accompanied by abdominal respiration, and two had a fever for one day only.One sow aborted at 109 days of gestation. The other seven sows, farrowing between 113 and 117 days of gestation, gave birth to numerous mummified, dead, and weak piglets. Of these seven, the mean number of piglets born dead to each sow was 4.6 and the mean number born alive was 7.7; 3.1 piglets per sow (40%) died within the first week.Lelystad virus was isolated from 31 piglets, which were born dead or died shortly after birth. Antibody was detected in precolostral blood samples or ascitic fluids of 23 piglets, a finding which demonstrated transplacental passage of the virus in six out of eight litters. We conclude that Lelystad virus is the causal agent of mystery swine disease. Since its aetiology is no longer a mystery, we propose the more appropriate name 'porcine epidemic abortion and respiratory syndrome (PEARS)'.