TUBULOVESICULAR PROCESSES EMERGE FROM TRANS-GOLGI CISTERNAE, EXTEND ALONG MICROTUBULES, AND INTERLINK ADJACENT TRANS-GOLGI ELEMENTS INTO A RETICULUM
TUBULOVESICULAR PROCESSES EMERGE FROM TRANS-GOLGI CISTERNAE, EXTEND ALONG MICROTUBULES, AND INTERLINK ADJACENT TRANS-GOLGI ELEMENTS INTO A RETICULUM
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DOI：
10.1016/0092-8674(90)90221-y
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发表时间：
1990-04-06
期刊：
影响因子：
64.5
通讯作者：
SMITH, SJ
中科院分区：
文献类型：
--
作者：
COOPER, MS;CORNELLBELL, AH;SMITH, SJ
利用电子增强荧光视频和激光共聚焦显微镜对nbd神经酰胺标记的星形胶质细胞活体高尔基体内的形态动力学和膜运输进行了成像。在延时记录中，观察到连续的管泡过程从反式高尔基元中出现，并以平均0.4 μ m/s的速率沿着微管延伸。此外，观察到离散的荧光颗粒从反式高尔基体中出现，随后沿微管以相当的速度迁移。通常，管状泡突形成稳定的连接，将相邻的反式高尔基体连接成一个广泛的网状结构。激光光漂白-恢复实验表明，管泡过程可以为膜脂在连接的反式高尔基元之间的扩散提供直接途径。这些结果表明，基于微管的转运和膜融合可以使细胞内邻近高尔基体的某些池膜相互连接。
Morphological dynamics and membrane transport within the living Golgi apparatus of astrocytes labeled with NBD-ceramide were imaged using both electronically enhanced fluorescence video and laser confocal microscopy. In time-lapse recordings, continuous tubulovesicular processes are observed to emerge from trans-Golgi elements and extend along microtubules at average rates of 0.4 .mu.m/s. In addition, discrete fluorescent particles are observed to emerge from the trans-Golgi and subsequently migrate along microtubules at comparable velocities. Frequently, tubulovesicular processes form stable connections tht interlink adjacent trans-Golgi elements into an extensive reticulum. Laser photobleaching-recovery experiments reveal that tubulovesicular processes can provide direct pathways for the diffusion of membrane lipids between joined trans-Golgi elements. These results suggest that microtubule-based transport and membrane fusion can operate to interconnect certain cisternal membranes of ajacent Golgi elements within the cell.