Single-cell technologies are revolutionizing the approach to rare cells.
Single-cell technologies are revolutionizing the approach to rare cells.
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DOI：
10.1038/icb.2015.106
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发表时间：
2016-03
影响因子：
4
通讯作者：
Lönnberg T
中科院分区：
文献类型：
--
作者：
Proserpio V;Lönnberg T
在过去的一段时间里，单细胞定量PCR、RNA和DNA测序等单细胞技术，以及最先进的飞行时间(CyTOF)细胞仪，使得从骨髓造血室到大脑的不同器官的亚成分得到了详细的分析。这些细粒度的分析突出了每个细胞室内的巨大异质性，揭示了以前未知的细胞亚群。在这篇综述中，我们分析了这种快速的技术进化如何提高了我们对生物过程的理解，特别是对免疫系统的稀有细胞的理解。
In the last lustrum single-cell techniques such as single-cell quantitative PCR, RNA and DNA sequencing, and the state-of-the-art cytometry by time of flight (CyTOF) mass cytometer have allowed a detailed analysis of the sub-composition of different organs from the bone marrow hematopoietic compartment to the brain. These fine-grained analyses have highlighted the great heterogeneity within each cell compartment revealing previously unknown subpopulations of cells. In this review, we analyze how this fast technological evolution has improved our understanding of the biological processes with a particular focus on rare cells of the immune system.