The DYT1-interacting proteins bHLH010, bHLH089 and bHLH091 are redundantly required for Arabidopsis anther development and transcriptome
The DYT1-interacting proteins bHLH010, bHLH089 and bHLH091 are redundantly required for Arabidopsis anther development and transcriptome
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DYT1 相互作用蛋白 bHLH010、bHLH089 和 bHLH091 对于拟南芥花药发育和转录组来说是多余的
DOI：
10.1111/tpj.12942
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发表时间：
2015
期刊：
影响因子：
7.2
通讯作者：
Chang Fang
中科院分区：
文献类型：
--
作者：
Zhu Engao;You Chenjiang;Wang Shuangshuang;Cui Jie;Niu Baixiao;Wang Yingxiang;Qi Ji;Ma Hong;Chang Fang
花药是开花植物的雄性生殖器官，由功能失调的TAPETUM1（DYT1）和流产的小孢子（AMS）编码的拟南芥bHLH转录因子是调控花药发育的复杂转录网络所必需的。bHLH蛋白影响这种不同基因表达的机制的知识是相当有限的。我们利用进化、遗传、形态学和转录组学的方法研究了三个最近重复的拟南芥bhlh基因bHLH010、bhlh089和bhlh091，并揭示了它们在花药发育中的冗余功能。这三个基因在拟南芥花药绒毡层中表达量较高；bhlh010、bhlh089和bhlh091位点的单突变体发育正常，但各种双和三组合逐渐表现出越来越多的缺陷花药表型（绒绒层形态异常、胼胝质变性延迟和花粉发育流产），表明它们在雄性生育能力中具有冗余功能。进一步的转录组学和分子分析表明，这三种蛋白的作用时间略晚于DYT1，并与DYT1形成蛋白复合物，从而影响了其他花药发育转录网络中许多DYT1靶基因的正确表达。本研究表明bHLH010、bHLH089和bHLH091共同对发育中的拟南芥花药的正常转录组起重要作用，可能与DYT1形成一个前馈回路。
The anther is the male reproductive organ of flowering plants, and the Arabidopsis bHLH transcription factors encoded byDYSFUNCTIONAL TAPETUM1(DYT1) andABORTED MICROSPORE(AMS) are required for control of the complex transcriptional networks regulating anther development. Knowledge of the mechanisms by which the bHLH proteins affect this diverse gene expression is quite limited. We examine here three recently duplicated ArabidopsisbHLHgenes,bHLH010,bHLH089andbHLH091, using evolutionary, genetic, morphological and transcriptomic approaches, and uncover their redundant functions in anther development. These three genes are relatively highly expressed in the tapetum of the Arabidopsis anther; single mutants at each of thebHLH010,bHLH089andbHLH091loci are developmentally normal, but the various double and triple combinations progressively exhibit increasingly defective anther phenotypes (abnormal tapetum morphology, delayed callose degeneration, and aborted pollen development), indicating their redundant functions in male fertility. Further transcriptomic and molecular analyses suggest that these three proteins act slightly later than DYT1, and also form protein complexes with DYT1, subsequently affecting the correct expression of many DYT1 target genes in the anther development transcriptional network. This study demonstrated that bHLH010, bHLH089 and bHLH091 together are important for the normal transcriptome of the developing Arabidopsis anther, possibly by forming a feed‐forward loop with DYT1.