Purification, chemical characterization and radical scavenging activities of alkali-extracted polysaccharide fractions isolated from the fruit bodies of Tricholoma matsutake
Purification, chemical characterization and radical scavenging activities of alkali-extracted polysaccharide fractions isolated from the fruit bodies of Tricholoma matsutake
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DOI：
10.1007/s11274-012-1232-x
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发表时间：
2013-05-01
影响因子：
4.1
通讯作者：
Sun, Xin
中科院分区：
文献类型：
--
作者：
Tong, Haibin;Liu, Ximing;Sun, Xin
松茸是我国的高级食用菌，是著名的食品和生物制药原料，引起了人们极大的兴趣。在以往的研究中，研究者认为水溶性多糖β-葡聚糖是T.松茸具有多种生物活性。本研究从黑木耳子实体中分离得到两个新的碱提多糖组分TM-APS-1和TM-APS-2。在A“TKA explorer色谱系统上，通过DEAE-纤维素和Sepharose CL-6 B柱对松茸进行分离。通过化学方法、GC、HPLC、清除DPPH自由基、超氧阴离子自由基、羟自由基、螯合能力等评价了它们的理化性质和清除自由基的能力。结果表明，TM-APS-1和TM-APS-2具有明显的抗氧化活性，且呈浓度依赖性。碱提多糖组分的含量与碱提多糖组分的含量呈线性关系。松茸可开发为具有抗氧化作用的新型功能性食品或药品。
Tricholoma matsutake, a high-class edible mushroom in China, has been regarded as famous foods and biopharmaceutical materials with a great deal of interest. In the previous investigations, researchers believe the water-soluble polysaccharide beta-glucan is the major active component of T. matsutake, which displays various biological activities. In the present study, two novel alkali-extracted polysaccharide fractions, TM-APS-1 and TM-APS-2, were isolated from the fruit bodies of T. matsutake by DEAE-cellulose and Sepharose CL-6B columns on A"TKA explorer chromatography system. Their chemical and physical characteristics and radical scavenging capacity were valuated, including chemical methods, GC, HPLC, scavenging activity against DPPH radicals, superoxide radicals, hydroxyl radicals, and chelating ability. The results showed that TM-APS-1 and TM-APS-2 exhibited significantly antioxidant activity at a concentration-dependent manner. The alkali-extracted polysaccharide fractions from T. matsutake can be developed to be novel functional food or pharmaceutical products with antioxidant effects.