SEQUENCE OF SOCKEYE-SALMON TYPE-1 AND TYPE-2 GROWTH-HORMONE GENES AND THE RELATIONSHIP OF RAINBOW-TROUT WITH ATLANTIC AND PACIFIC SALMON
SEQUENCE OF SOCKEYE-SALMON TYPE-1 AND TYPE-2 GROWTH-HORMONE GENES AND THE RELATIONSHIP OF RAINBOW-TROUT WITH ATLANTIC AND PACIFIC SALMON
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DOI：
10.1139/f93-195
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发表时间：
1993-08-01
影响因子：
2.4
通讯作者：
DEVLIN, RH
中科院分区：
文献类型：
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作者：
DEVLIN, RH
已从红鲑鱼（Oncorhynchus nerka）中分离出两种类型的生长激素基因，并确定了它们的完整核苷酸序列。这些基因编码 210 个氨基酸的蛋白质，与其他鲑鱼和鱼类的生长激素非常相似。这两个基因可能源于基因复制事件，该事件在鲑鱼中产生了四倍体条件，并且在其编码区中高度保守。自基因重复事件以来，非编码区的序列已出现约 18% 的分歧，并显示出大量的删除和/或插入。从太平洋鲑鱼中分离这两个基因可以将它们的序列与虹鳟鱼和大西洋鲑鱼的生长激素基因进行比较。结果表明，虹鳟鱼与太平洋鲑鱼比与大西洋鲑鱼更相似，并表明当红鲑鱼和虹鳟鱼是共同繁殖种群的一部分时，大西洋鲑鱼与太平洋鲑鱼分化。这些结果支持最近将虹鳟鱼从鲑鱼属重新分类为虹鳟鱼属。
Two types of growth hormone genes have been isolated from sockeye salmon (Oncorhynchus nerka) and their complete nucleotide sequence determined. The genes encode proteins of 210 amino acids and show considerable similarity to growth hormones characterized in other salmonids and fishes. The two genes presumably arose from a gene duplication event that generated the tetraploid condition in salmonids and are highly conserved in their coding regions. The sequences have diverged approximately 18% in noncoding regions since the gene duplication event and show numerous deletions and/or insertions. Isolation of these two genes from a Pacific salmon allows comparison of their sequences to growth hormone genes characterized from rainbow trout and from Atlantic salmon. The results indicate that rainbow trout is more similar to Pacific than to Atlantic salmon and suggest that Atlantic salmon diverged from Pacific salmonids at a time when sockeye and rainbow trout were part of a common breeding population. These results support the recent reclassification of rainbow trout from the genus Salmo to Oncorhynchus.