Genetic differentiation of continental and island populations of Bombus terrestris (Hymenoptera: Apidae) in Europe
Genetic differentiation of continental and island populations of Bombus terrestris (Hymenoptera: Apidae) in Europe
复制标题
DOI：
10.1111/j.1365-294x.1996.tb00288.x
复制
发表时间：
1996-02-01
影响因子：
4.9
通讯作者：
Scholl, A
中科院分区：
文献类型：
--
作者：
Estoup, A;Solignac, M;Scholl, A
利用10个微卫星位点和线粒体COII基因部分序列研究了B群体的遗传分化。terrestris，一种因其高价值作物授粉而受到关注的熊蜂。该分析包括来自欧洲大陆的8个种群，5个来自地中海岛屿（共6个亚种），1个来自特内里费岛（最初被描述为B的颜色形式）。terrestris，但最近被认为是一个独立的种，B. canariensis）。10个微卫星位点中有8个在大多数群体中表现出高水平的多态性。在B。结果表明，在20个群体中，每个多态位点检测到的等位基因总数为3 ~ 16个，等位基因多样性为3.8 ± 0.5 ~ 6.5 ± 1.4，平均杂合度为0.41 ± 0.09 ~ 0.65 ± 0.07。B。与B的大陆和岛屿群体相比，canariensis显示出显著较低的平均计算杂合性（0.12 +/- 0.08）和观察到的等位基因多样性（1.5 +/- 0.04）。terrestris。B群体间无明显分化。产于欧洲大陆。与此相反，岛屿种群都是显着的，其中大多数人强烈分化的大陆种群。B。陆生动物线粒体DNA的核苷酸多样性较低，大陆种群、岛屿种群和所有种群的核苷酸多样性分别为0.18% +/-0.07%、0.20% +/- 0.04%和0.27% +/- 0.04%。在特内里费岛人群中发现的唯一单倍型与最常见的大陆单倍型B的单核苷酸取代不同。terrestris。这种情况与第勒尼安群岛种群的情况相同，与B种群的情况大不相同。Lucorum（terrestris和Lucorum mtDNA之间有15个替换）对B的种地位提出了质疑。加那利群岛。特内里费与B之间的遗传距离较大。terrestris人口估计从微卫星数据的结果，最有可能的是，从一个严重的瓶颈在加那利群岛的人口。微卫星和线粒体DNA数据呼吁保护岛屿种群的B。terrestris反对进口用作农作物授粉者的外国来源的熊蜂。
Ten microsatellite loci and a partial sequence of the COII mitochondrial gene were used to investigate genetic differentiation in B. terrestris, a bumble bee of interest for its high-value crop pollination. The analysis included eight populations from the European continent, five from Mediterranean islands (six subspecies altogether) and one from Tenerife (initially described as a colour form of B. terrestris but recently considered as a separate species, B. canariensis). Eight of the 10 microsatellite loci displayed high levels of polymorphism in most populations. In B. terrestris populations, the total number of alleles detected per polymorphic locus ranged from 3 to 16, with observed allelic diversify from 3.8 +/- 0.5 to 6.5 +/- 1.4 and average calculated heterozygosities from 0.41 +/- 0.09 to 0.65 +/- 0.07. B. canariensis showed a significantly lower average calculated heterozygosity (0.12 +/- 0.08) and observed allelic diversity (1.5 +/- 0.04) as compared to both continental and island populations of B. terrestris. No significant differentiation was found among populations of B. terrestris from the European continent. In contrast, island populations were all significantly and most of them strongly differentiated from continental populations. B. terrestris mitochondrial DNA is characterized by a low nucleotide diversity: 0.18% +/- 0.07%, 0.20% +/- 0.04% and 0.27% +/- 0.04% for the continental populations, the island populations and all populations together, respectively. The only haplotype found in the Tenerife population differs by a single nucleotide substitution from the most common continental haplotype of B. terrestris. This situation, identical to that of Tyrrhenian islands populations and quite different from that of B. lucorum (15 substitutions between terrestris and lucorum mtDNA) casts doubts on the species status of B. canariensis. The large genetic distance between the Tenerife and B. terrestris populations estimated from microsatellite data result, most probably, from a severe bottleneck in the Canary island population. Microsatellite and mitochondrial DNA data call for the protection of the island populations of B. terrestris against importation of bumble bees of foreign origin which are used as crop pollinators.