Retinal blood flow abnormalities following six months of hyperglycemia in the Ins2(Akita) mouse.
Retinal blood flow abnormalities following six months of hyperglycemia in the Ins2(Akita) mouse.
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DOI：
10.1016/j.exer.2012.03.003
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发表时间：
2012-05
影响因子：
3.4
通讯作者：
Harris, Norman R.
中科院分区：
文献类型：
--
作者：
Wright, William S.;Yadav, Amit Singh;McElhatten, Robert M.;Harris, Norman R.
本研究的目的是表征糖尿病Ins 2（秋田）小鼠中高血糖6个月后存在的微血管血流异常和氧合变化。先前的研究表明，啮齿动物在高血糖症的前几周视网膜血流量减少，类似于人类糖尿病早期阶段的减少。然而，小鼠视网膜中的这种变化是否会持续到糖尿病的最初几周之后，还有待确定，因为视网膜氧合流量减少的潜在后果也是如此。在这项研究中，雄性Ins 2（秋田）和年龄匹配的C57 BL/6（非糖尿病）小鼠维持了6个月的时间，在此期间使用活体显微镜来测量视网膜血管直径、血细胞速度、血管壁剪切速率、血流速率和瞬时毛细血管闭塞。此外，缺氧的存在进行了评估，使用氧敏感探针pimonidazole。糖尿病视网膜微血管显示红细胞速度（30%，p<0.001）、切变率（25%，p<0.01）和流速（40%，p<0.001）降低。此外，在糖尿病小鼠中观察到短暂的毛细血管流动停止，但在非糖尿病小鼠中很少。然而，通过哌莫硝唑测定法测定，没有观察到视网膜缺氧的改变，这表明视网膜血流减少可能是由视网膜氧利用率降低决定的。
The aim of this study was to characterize the microvascular flow abnormalities and oxygenation changes that are present following six months of hyperglycemia in the diabetic Ins2(Akita) mouse. Previous studies have shown decreased retinal blood flow in the first several weeks of hyperglycemia in rodents, similar to the decreases seen in the early stages of human diabetes. However, whether this alteration in the mouse retina continues beyond the initial weeks of diabetes has yet to be determined, as are the potential consequences of the decreased flow on retinal oxygenation. In this study, male Ins2(Akita) and age-matched C57BL/6 (non-diabetic) mice were maintained for a period of six months, at which time intravital microscopy was used to measure retinal blood vessel diameters, blood cell velocity, vascular wall shear rates, blood flow rates, and transient capillary occlusions. In addition, the presence of hypoxia was assessed using the oxygen-sensitive probe pimonidazole. The diabetic retinal microvasculature displayed decreases in red blood cell velocity (30%, p<0.001), shear rate (25%, p<0.01), and flow rate (40%, p<0.001). Moreover, transient capillary stoppages in flow were observed in the diabetic mice, but rarely in the non-diabetic mice. However, no alterations were observed in retinal hypoxia as determined by a pimonidazole assay, suggesting the possibility that the decreases seen in retinal blood flow may be dictated by a decrease in retinal oxygen utilization.
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影响因子：
3.7
作者：
Mowat FM;Luhmann UF;Smith AJ;Lange C;Duran Y;Harten S;Shukla D;Maxwell PH;Ali RR;Bainbridge JW
通讯作者：
Bainbridge JW
影响因子：
2.4
作者：
Lee, Seungjun;Harris, Norman R.
通讯作者：
Harris, Norman R.
影响因子：
4.2
作者：
Durham, Jennifer T.;Herman, Ira M.
通讯作者：
Herman, Ira M.
影响因子：
4.2
作者：
Clermont, AC;Aiello, LP;Bursell, SE
通讯作者：
Bursell, SE
影响因子：
13.6
作者：
Faulhaber-Walter, Robert;Chen, Limeng;Schnermann, Jurgen
通讯作者：
Schnermann, Jurgen