Evaluation of the anti-inflammatory, analgesic and antipyretic activities of the natural polyphenol chlorogenic acid
Evaluation of the anti-inflammatory, analgesic and antipyretic activities of the natural polyphenol chlorogenic acid
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DOI：
10.1248/bpb.29.2236
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发表时间：
2006-11-01
影响因子：
2
通讯作者：
Emilia Petto de Souza, Gloria
中科院分区：
文献类型：
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作者：
David dos Santos, Michel;Camila Almeida, Maria;Emilia Petto de Souza, Gloria
酚类化合物在植物界中数量众多且普遍存在，尤其存在于促进健康的食品中。流行病学证据表明，食用富含多酚的食物可以降低癌症、冠心病和炎症的发病率。绿原酸（Chlorogenic Acid，CGA）是人类膳食中含量最丰富的多酚类化合物之一。从体内和体外实验中获得的数据表明，CGA主要表现出抗氧化和抗癌活性。然而，迄今为止，CGA对炎症反应以及相关疼痛和发热过程的影响较少被探索。因此，本研究旨在评价CGA在大鼠中的抗炎、镇痛和解热活性。与对照相比，剂量为50和100 mg/kg的CGA在实验程序的第2小时开始抑制角叉菜胶诱导的爪水肿。此外，在50和100 mg/kg剂量下，CGA还抑制福尔马林诱导的疼痛试验后期的退缩次数。这些活性可能来源于CGA在参与这些反应的炎性介质的外周合成/释放中的抑制作用。另一方面，即使在最高试验剂量（200 mg/kg）下，CGA也不能抑制脂多糖（LPS）诱导的大鼠发热反应。为了阐明CGA抗炎和镇痛作用的真正靶点，还需要进行额外的实验。
Phenolic compounds are numerous and ubiquitous in the plant kingdom, being particularly present in health-promoting foods. Epidemiological evidences suggest that the consumption of polyphenol-rich foods reduces the incidence of cancer, coronary heart disease and inflammation. Chlorogenic acid (CGA) is one of the most abundant polyphenol compounds in human diet. Data obtained from in vivo and in vitro experiments show that CGA mostly presents antioxidant and anti-carcinogenic activities. However, the effects of CGA on the inflammatory reaction and on the related pain and fever processes have been explored less so far. Therefore, this study was designed to evaluate the anti-inflammatory, antinociceptive and antipyretic activities of CGA in rats. In comparison to control, CGA at doses 50 and 100 mg/kg inhibited carrageenin-induced paw edema beginning at the 2nd hour of the experimental procedure. Furthermore, at doses 50 and 100 mg/kg CGA also inhibited the number of flinches in the late phase of formalin-induced pain test. Such activities may be derived from the inhibitory action of CGA in the peripheral synthesis/release of inflammatory mediators involved in these responses. On the other hand, even at the highest tested dose (200 mg/kg), CGA did not inhibit the febrile response induced by lipopolysaccharide (LPS) in rats. Additional experiments are necessary in order to clarify the true target for the anti-inflammatory and analgesic effects of CGA.