Knockdown of ectodysplasin-A receptor-associated adaptor protein exerts a tumor-suppressive effect in tongue squamous cell carcinoma cells
Knockdown of ectodysplasin-A receptor-associated adaptor protein exerts a tumor-suppressive effect in tongue squamous cell carcinoma cells
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DOI：
10.3892/etm.2020.8578
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发表时间：
2020-05-01
影响因子：
2.7
通讯作者：
Meng, Jian
中科院分区：
文献类型：
--
作者：
Li, Meng;Bai, Yu-Ting;Meng, Jian
舌鳞状细胞癌（TSCC）是口腔癌中一种常见的恶性肿瘤，死亡率和发病率都很高。ectodysplasin- a受体相关接头蛋白（EDARADD）是一种含有死亡结构域的接头蛋白，可与TNF家族配体ectodysplasin a受体相互作用。众所周知，EDARADD对外胚层衍生组织（如头发和牙齿）的发育有影响。EDARADD的表达也与黑色素瘤的发生有关。然而，EDARADD在TSCC中的作用尚不清楚。本研究的目的是探讨EDARADD是否在TSCC的生物学功能中发挥作用。免疫组化检测EDARADD在TSCC组织及邻近正常组织中的表达。EDARADD在体外的TSCC细胞系中被特异性慢病毒敲除。采用逆转录-定量PCR检测EDARADD基因表达水平及基因敲低效果，western blotting检测EDARADD蛋白表达及Bcl-2、MYC、NF-kappa Bp65表达水平。此外，通过MTT实验、集落形成实验和细胞凋亡实验检测EDARADD基因敲除对TSCC细胞的影响。先前的一项研究表明，大多数被检测的TSCC组织具有高表达的EDARADD。EDARADD在mRNA和蛋白水平上的表达均显著降低(P
Tongue squamous cell carcinoma (TSCC) is a common malignancy in oral cancer with a high mortality and morbidity. The ectodysplasin-A receptor-associated adaptor protein (EDARADD) is a death domain-containing adaptor protein that interacts with the TNF family ligand ectodysplasin A receptor. It is known that EDARADD has an effect on the development of ectodermal derivative tissues, such as hair and teeth. EDARADD expression is also associated with the development of melanoma. However, the role of EDARADD in TSCC remains unknown. The aim of the present investigation was to explore whether EDARADD plays a role in the biological function of TSCC. Immunohistochemistry was used to measure the expression of EDARADD in TSCC tissues and adjacent normal tissue. EDARADD was knocked down in a TSCC cell line in vitro using a specific lentivirus. The expression level of the EDARADD gene and the efficacy of gene knockdown were evaluated by reverse transcription-quantitative PCR, while EDARADD protein expression and the expression levels of Bcl-2, MYC and NF-kappa Bp65 were determined by western blotting. Additionally, MTT assays, colony formation assays and apoptosis assays were carried out to examine the effect of EDARADD knockdown on the TSCC cells. A previous study showed that the majority of the TSCC tissues that were tested had high EDARADD expression. The expression of EDARADD both at mRNA and protein levels was significantly lower (P