Single cell oil (SCO) production by Mortierella isabellina grown on high-sugar content media
Single cell oil (SCO) production by Mortierella isabellina grown on high-sugar content media
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DOI：
10.1016/j.biortech.2004.02.016
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发表时间：
2004-12-01
影响因子：
11.4
通讯作者：
Aggelis, G
中科院分区：
文献类型：
--
作者：
Papanikolaou, S;Komaitis, M;Aggelis, G
氮限制培养基中培养的深黄被孢霉表现出显著的细胞生长（高达35.9 g/l）和高葡萄糖摄取，即使在培养基中具有高初始糖浓度（例如100 g/l）。在氮耗尽后，在真菌菌丝体内积累了显著的脂肪量（50- 55%，干生物质中的wt/wt油），导致18.1g/l培养基的显著单细胞油产量。总干生物量和脂质产量的值大大增加（0.34和0.17克分别每克葡萄糖消耗）。所产生的微生物脂质含有浓度为3.5 +/-1.0%（wt/wt）的γ-亚麻酸（GLA），其对应于每克干微生物质量16-19 mg GLA和每升培养基0.801 g GLA的最大浓度。(C)2004 Elsevier Ltd.保留所有权利。
Mortierella isabellina cultivated in nitrogen-limited media presented remarkable cell growth (up to 35.9 g/l) and high glucose uptake even with high initial sugar concentrations (e.g. 100 g/l) in media. After nitrogen depletion, significant fat quantities were accumulated inside the fungal mycelia (50-55%, wt/wt oil in dry biomass), resulting in a notable single cell oil production of 18.1 g/l of culture medium. Total dry biomass and lipid yields presented greatly increased values (0.34 and 0.17 g respectively per gram of glucose consumed). The microbial lipid produced contained gamma-linolenic acid (GLA) at a concentration of 3.5 +/- 1.0%, wt/wt, which corresponded to 16-19 mg GLA per gram of dry microbial mass and a maximum concentration of 0.801 g GLA per liter of culture medium. (C) 2004 Elsevier Ltd. All rights reserved.