Antibodies against neuronal nicotinic receptor subtypes in neurological disorders
Antibodies against neuronal nicotinic receptor subtypes in neurological disorders
复制标题
DOI：
10.1016/s0165-5728(99)00145-9
复制
发表时间：
2000-01-03
影响因子：
3.3
通讯作者：
Gotti, C
中科院分区：
文献类型：
--
作者：
Balestra, B;Moretti, M;Gotti, C
重症肌无力（MG）患者具有针对肌肉烟碱乙酰胆碱受体（mAChR）的抗体，这是导致其肌无力的原因：但一些MG和其他神经免疫性疾病患者具有自主神经症状。我们的特点是神经元形式的乙酰胆碱受体（nAChRs）到两个神经母细胞瘤细胞系和开发免疫沉淀试验，以测试抗体的α 7-和α 3-含nAChR亚型，存在于自主神经节。然后，我们测试了70名MG患者的血清样本，38名其他神经系统疾病的受试者，30名健康人，这两种形式的神经元AChR亚型的抗体。我们使用从用I-125-α银环蛇毒素（α Bgtx）标记的人成神经细胞瘤IMR 32细胞系提取的α 7亚型，和从用H-3-Epibatidine（Epi）标记的人成神经细胞瘤SY 5 Y细胞系提取的含α 3亚型。9名受试者（5名MG、1名GBS、1名CIPD和2名LEMS）为α 7亚型阳性; 4名为含α 3亚型阳性（2名MG患者、1名LEMS和同一名GBS患者）。肌乙酰胆碱受体抗体检测阴性的MG患者均为阴性。血清抗体α 7或α 3含神经AChRs的患者表现出一系列的临床特征，包括自主神经症状和胸腺瘤在两个MG患者。这些结果表明，MG和其他免疫介导的疾病的患者可以有神经元AChR的抗体，这些可能有助于疾病的临床特征。(C)2000 Elsevier Science B. V.保留所有权利。
Patients with myasthenia gravis (MG) have antibodies to the muscle nicotinic acetylcholine receptor (mAChR) which are responsible for their muscle weakness: but some patients with MG and other neuroimmunological disorders have autonomic symptoms. We characterised the neuronal forms of AChRs (nAChRs) into two neuroblastoma cell lines and developed immunoprecipitation assays to test for antibodies to the alpha 7- and alpha 3-containing nAChR subtypes, present in the autonomic ganglia. We then tested 70 sera samples from MG patients, 38 from subjects with other neurological diseases, and 30 from healthy individuals, for antibodies to these two forms of neuronal AChR subtypes. We used the alpha 7 subtype extracted from the human neuroblastoma IMR32 cell line labeled with I-125-alpha Bungarotoxin (alpha Bgtx), and the alpha 3-containing subtype extracted from the human neuroblastoma SY5Y cell line labeled with H-3-Epibatidine (Epi). Nine subjects (five MG, one GBS, one CIPD and two LEMS) were positive for the a7 subtype; and four for the alpha 3-containing subtype (two MG patients, one LEMS and the same GBS patient). None of the MG patients with undetectable levels of antibodies against muscle AChR were positive. The patients with serum antibodies to alpha 7 or alpha 3-containing neuronal AChRs showed a range of clinical features including autonomic symptoms and thymoma in two MG patients. These results indicate that patients with MG and other immune-mediated disorders can have antibodies to neuronal AChRs, and that these may contribute to the clinical characteristics of the diseases. (C) 2000 Elsevier Science B.V. All rights reserved.