Combinations of monoclonal antibodies distinguish mesenchymal, myogenic, and chondrogenic precursors of the developing chick embryo.
Combinations of monoclonal antibodies distinguish mesenchymal, myogenic, and chondrogenic precursors of the developing chick embryo.
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单克隆抗体的组合可区分发育中鸡胚的间充质、肌源和软骨前体。
DOI：
10.1016/0012-1606(88)90056-5
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发表时间：
1988
影响因子：
2.7
通讯作者：
Horwitz,A
中科院分区：
文献类型：
--
作者：
George-Weinstein,M;Decker,C;Horwitz,A
用单抗(MAb)作为探针，检测发育中的雏鸡肢体非终末分化细胞表面的分子差异。通过对培养的胸肌和肢芽细胞以及各种胚胎(15-37期)和新生儿组织的冰冻切片进行免疫荧光定位，确定了单抗的特异性。用荧光激活细胞分选法分离单抗阳性和阴性细胞亚群，并在体外检测其发育潜能。用CSAT单抗标记致密的体节、侧板中胚层和早期肢芽的细胞。皮肤部和肢芽的肌源性前体用CSAT和L4单抗标记。用CSAT、L4和C5单抗标记硬化体和肢芽的软骨前体。这些前体细胞不同于CSAT和C1MAb标记的成纤维细胞。肌肉和软骨的分化和成熟伴随着单抗标记模式的改变。这些结果表明，这些单抗的组合可用于区分间充质前体、肌源性前体和软骨源性前体，确定它们在发育过程中的起源位置，并分离胚胎细胞亚群。
Monoclonal antibodies (MAbs) were used as probes for molecular differences in the surfaces of nonterminally differentiated cells of the developing chick limb. The specificity of the MAbs was determined by immunofluorescent localization performed on cultured breast muscle and limb bud cells and cryosections of a variety of embryonic (stages 15–37) and neonatal tissues. Subpopulations of MAb-positive and -negative cells were isolated by fluorescence-activated cell sorting and their developmental potential was assessedin vitro. Cells of the compacted somite, lateral plate mesoderm, and early limb bud were labeled with the CSAT MAb. Myogenic precursors of the dermatome and limb bud were labeled with the CSAT and L4 MAbs. Chondrogenic precursors of the sclerotome and limb bud were labeled with the CSAT, L4, and C5 MAbs. These precursors were distinguished from fibroblasts which were labeled with the CSAT and C1 MAbs. The differentiation and maturation of muscle and cartilage were accompanied by alterations in the labeling patterns of the MAbs. These results indicate that combinations of these MAbs can be used to distinguish mesenchymal, myogenic, and chondrogenic presursors, identify their site of origin during development, and isolate subpopulations of embryonic cells.