Assessment of the Chemical Diversity and Potential Toxicity of Benthic Cyanobacterial Blooms in the Lagoon of Moorea Island (French Polynesia)
Assessment of the Chemical Diversity and Potential Toxicity of Benthic Cyanobacterial Blooms in the Lagoon of Moorea Island (French Polynesia)
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DOI：
10.3390/jmse8060406
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发表时间：
2020-06-01
影响因子：
2.9
通讯作者：
Roue, Melanie
中科院分区：
文献类型：
--
作者：
Bonnard, Isabelle;Bornancin, Louis;Roue, Melanie
在过去的几十年里，珊瑚礁和热带泻湖中底栖蓝藻爆发的频率明显增加，部分原因可能是全球变化和人为活动。在对Moorea岛（法属波利尼西亚）泻湖中繁殖的海洋底栖蓝藻进行调查的框架内，共收集到15次藻华，主要涉及3种：anabaena sp.1、Lyngbya majuscula和Hydrocoleum majus-B。通过高效液相色谱结合紫外检测和质谱（HPLC-UV-MS）分析获得的化学指纹图谱显示出高度的物种特异性。Anabaena sp.1的化学特征是由三种主要的泻药素家族的环脂肽组成，而L. majuscula的化学特征是由tiahuramide、trungapeptin和丝氨酸醇衍生的malyngamides组成的复杂混合物。使用盐蒿和小鼠神经母细胞瘤细胞（CBA-N2a）细胞毒性测定法对这些蓝藻样本进行的毒性筛选分析没有显示出任何毒性，没有达到值得对公共卫生进行风险评估的程度。然而，在法属波利尼西亚的珊瑚礁上，Lyngbya、Hydrocoleum、Anabaena或其他底栖蓝藻的大量繁殖明显增加，鼓励了对其增殖的监测和相关危害的潜在评估的特别监测计划的实施。
In the last decades, an apparent increase in the frequency of benthic cyanobacterial blooms has occurred in coral reefs and tropical lagoons, possibly in part because of global change and anthropogenic activities. In the frame of the survey of marine benthic cyanobacteria proliferating in the lagoon of Moorea Island (French Polynesia), 15 blooms were collected, mainly involving three species-Anabaena sp.1, Lyngbya majuscula and Hydrocoleum majus-B. Their chemical fingerprints, obtained through high performance liquid chromatography combined with UV detection and mass spectrometry (HPLC-UV-MS) analyses, revealed a high extent of species-specificity. The chemical profile of Anabaena sp.1 was characterized by three major cyclic lipopeptides of the laxaphycin family, whereas the one of L. majuscula was characterized by a complex mixture including tiahuramides, trungapeptins and serinol-derived malyngamides. Toxicity screening analyses conducted on these cyanobacterial samples using Artemia salina and mouse neuroblastoma cell-based (CBA-N2a) cytotoxic assays failed to show any toxicity to a degree that would merit risk assessment with regard to public health. However, the apparently increasing presence of blooms of Lyngbya, Hydrocoleum, Anabaena or other benthic cyanobacteria on coral reefs in French Polynesia encourages the implementation of ad hoc monitoring programs for the surveillance of their proliferation and potential assessment of associated hazards.