Analysis of the full-length genome of a hepatitis E virus isolate obtained from a wild boar in Japan that is classifiable into a novel genotype
Analysis of the full-length genome of a hepatitis E virus isolate obtained from a wild boar in Japan that is classifiable into a novel genotype
复制标题
DOI：
10.1099/vir.0.029470-0
复制
发表时间：
2011-04-01
影响因子：
3.8
通讯作者：
Okamoto, Hiroaki
中科院分区：
文献类型：
--
作者：
Takahashi, Masaharu;Nishizawa, Tsutomu;Okamoto, Hiroaki
在全国范围内对450头野猪进行戊型肝炎病毒（HEV）感染调查时，在2005年11月至2010年3月间在日本捕获的野猪中，我们发现了16头野猪，（3.6%）持续HEV感染：11头具有基因型3 HEV，4头具有基因型4 HEV，剩余的公猪感染了未识别基因型的HEV（命名为wbJOY_06）。对整个wbJOY_06基因组进行测序，发现其包含7246个nt，不包括poly（A）尾。在整个基因组上，wbJOY_06分离株与来自人、猪、野猪、鹿、猫鼬和兔的已知基因型1-4 HEV分离株（n=145）高度不同，差异为22.6- 27.7%，与大鼠HEV分离株（n=2）高度不同，差异为46.0- 46.2%，与禽类HEV分离株（n=5）高度不同，差异为52.5-53.1%。Simplot分析显示基因型1-4的现有HEV毒株之间没有显著的重组。因此，我们认为wbJOY_06分离株是先前未鉴定的基因型的第一个成员。
While performing a nationwide survey of hepatitis E virus (HEV) infection among 450 wild boars (Sus scrofa leucomystax) that had been captured in Japan between November 2005 and March 2010, we found 16 boars (3.6%) with ongoing HEV infection: 11 had genotype 3 HEV, four had genotype 4 HEV and the remaining boar was infected with HEV of an unrecognized genotype (designated wbJOY_06). The entire wbJOY_06 genome was sequenced and was found to comprise 7246 nt excluding the poly(A) tail. The wbJOY_06 isolate was highly divergent from known genotype 1-4 HEV isolates derived from humans, swine, wild boars, deer, mongoose and rabbits (n=145) by 22.6-27.7%, rat HEV isolates (n=2) by 46.0-46.2%, and avian HEV isolates (n=5) by 52.5-53.1% over the entire genome. A Simplot analysis revealed no significant recombination between the existing HEV strains of genotypes 1-4. Therefore, we propose that the wbJOY_06 isolate is the first member of a previously unidentified genotype.