High-peak-power 918-nm laser light source based two-photon spinning-disk microscopy for green fluorophores
High-peak-power 918-nm laser light source based two-photon spinning-disk microscopy for green fluorophores
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DOI：
10.1016/j.bbrc.2020.05.213
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发表时间：
2020-08-20
影响因子：
3.1
通讯作者：
Nemoto, Tomomi
中科院分区：
文献类型：
--
作者：
Otomo, Kohei;Goto, Ai;Nemoto, Tomomi
使用配备转盘扫描单元的双光子显微镜对活体标本进行高速成像。通常，需要高峰值功率激光光源在数百个焦点处同时引发双光子激发过程，从而产生可激发荧光团的局限性。因此，高峰值功率的基于钕的 918 nm 激光光源被用于对最流行的荧光团（绿色荧光蛋白）进行活体成像。结果，所提出的系统获得的荧光信号比使用传统锁模钛蓝宝石激光光源获得的荧光信号亮大约 30 倍。此外，该系统还可视化了表达G-CaMP7的活小鼠（体积为6000万μm（3））中胰腺腺泡细胞激动剂刺激的四维（xyz-t）钙反应。 (C) 2020 作者。由爱思唯尔公司出版
High-speed imaging of living specimen was performed using two-photon microscopy equipped with a spinning-disk scanning unit. Typically, a high-peak-power laser light source is needed to simultaneously induce two-photon excitation processes at several hundred focal points, generating the limitations of excitable fluorophores. Therefore, a high-peak-power neodymium-based 918-nm laser light source was used for intravital imaging of the most popular fluorophores, green fluorescent proteins. As a result, the proposed system obtained approximately 30 times brighter fluorescent signal than that obtained using a conventional mode-locked titanium:sapphire laser light source. Furthermore, the system visualized four-dimensional (xyz-t) calcium responses of pancreatic acinar cells agonist stimulations in the living G-CaMP7-expressing mouse with 60 million mu m(3) volume. (C) 2020 The Authors. Published by Elsevier Inc.