Theoretical Considerations for Optimizing the Use of Optogenetics with Complex Behavior.
Theoretical Considerations for Optimizing the Use of Optogenetics with Complex Behavior.
复制标题
优化复杂行为光遗传学应用的理论考虑。
DOI：
10.1002/cpz1.836
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发表时间：
2023
期刊：
影响因子：
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通讯作者：
LaLumiere,RyanT
中科院分区：
文献类型：
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作者：
Glickman,Bess;LaLumiere,RyanT
光遗传学方法使研究人员能够解决以前无法回答的关于行为的复杂问题。然而，由于光遗传过程涉及多个维度的大参数空间，因此将这些参数与所研究的行为结合起来考虑是至关重要的。在这里，我们讨论了通过确定关键的实验设计考虑因素来优化具有复杂行为的光遗传方法的策略，包括频率专一性、时间精度、活动控制的光遗传学、刺激模式和细胞类型专一性。我们强调了在操纵光遗传学实验的每一个因素时所要考虑的潜在限制或理论考虑。这篇综述强调了优化光遗传学研究设计的重要性，以加强关于行为神经科学的结论。©2023作者。由威利期刊有限责任公司出版的现行协议。
Optogenetic approaches have allowed researchers to address complex questions about behavior that were previously unanswerable. However, as optogenetic procedures involve a large parameter space across multiple dimensions, it is crucial to consider such parameters in conjunction with the behaviors under study. Here, we discuss strategies to optimize optogenetic approaches with complex behavior by identifying critical experimental design considerations, including frequency specificity, temporal precision, activity‐controlled optogenetics, stimulation pattern, and cell‐type specificity. We highlight potential limitations or theoretical considerations to be made when manipulating each of these factors of optogenetic experiments. This overview emphasizes the importance of optimizing optogenetic study design to enhance the conclusions that can be drawn about the neuroscience of behavior. © 2023 The Authors. Current Protocols published by Wiley Periodicals LLC.