Modulation of a thermoregulated type VI secretion system by AHL-dependent Quorum Sensing in Yersinia pseudotuberculosis
Modulation of a thermoregulated type VI secretion system by AHL-dependent Quorum Sensing in Yersinia pseudotuberculosis
复制标题
AHL 依赖性群体感应对假结核耶尔森菌温度调节 VI 型分泌系统的调节
DOI：
10.1007/s00203-011-0680-2
复制
发表时间：
2011-05-01
影响因子：
2.8
通讯作者：
Yuan, Zhiming
中科院分区：
文献类型：
--
作者：
Zhang, Weipeng;Xu, Shengjuan;Yuan, Zhiming
VI型分泌系统（T6 SS）是在许多革兰氏阴性细菌病原体中发现的一种新型分泌系统，它似乎受到不同调节机制的严格调节。在本研究中，我们确定了4个T6 SS集群在假结核耶尔森氏菌，并证明他们的差异温度调节。其中，T6 SS 4在26°C下优先表达，其表达具有生长期依赖性，并受群体感应调节。YpsI和YtbI阿勒酶都对T6 SS 4的正调控有贡献，而YpsI合酶起主要作用，因为T6 SS 4表达在ypsI突变株中强烈降低，但在ytbI突变株中较弱。此外，我们提供的证据表明，外源性添加不同的合成AHLs补充T6 SS 4的表达在不同的效率在ypsIytbI双突变株，表明C6-HSL对T6 SS 4的表达有拮抗作用。这是第一个研究表明，T6 SS的表达是精确调控的温度，生长阶段，和AHL依赖的群体感应系统在Y。假结核
The type VI secretion system (T6SS) is a novel secretion system found in many Gram-negative bacterial pathogens, which appears to be tightly regulated by different regulatory mechanisms. In the present study, we identified 4 T6SS clusters in Yersinia pseudotuberculosis and demonstrated that they were differentially thermoregulated. Among them, T6SS4 was preferentially expressed at 26°C, and its expression was growth phase dependent and subject to quorum sensing regulation. Both YpsI and YtbI AHL synthases contributed to the positive regulation of T6SS4, whereas YpsI synthase played the major role as T6SS4 expression was reduced strongly in the ypsI mutant strain but weakly in the ytbI mutant strain. Moreover, we provided evidence that exogenous addition of different synthetic AHLs complemented T6SS4 expression in different efficiencies in an ypsIytbI double mutant strain, suggesting C6-HSL had an antagonistic effect on T6SS4 expression. This is the first study demonstrating that the expression of T6SS is precisely regulated by temperature, growth phase, and AHL-dependent quorum sensing systems in Y. pseudotuberculosis.