The expression of EMP1 is downregulated in oral squamous cell carcinoma and possibly associated with tumour metastasis
The expression of EMP1 is downregulated in oral squamous cell carcinoma and possibly associated with tumour metastasis
复制标题
EMP1在口腔鳞状细胞癌中表达下调并可能与肿瘤转移相关
DOI：
10.1136/jcp.2010.082404
复制
发表时间：
2011-01-01
影响因子：
3.4
通讯作者：
Chen, Wan-tao
中科院分区：
文献类型：
--
作者：
Zhang, Jun;Cao, Wei;Chen, Wan-tao
目的探讨EMP 1在口腔鳞状细胞癌（OSCC）组织中的表达及其与临床指标的相关性。方法采用逆转录-聚合酶链反应（RT-PCR）检测18例口腔鳞癌组织及相应癌旁正常组织中EMP 1 mRNA的表达。另选取45对口腔鳞癌组织，采用定量RT-PCR（qRT-PCR）检测EMP 1 mRNA水平。采用免疫组化法检测60例口腔鳞癌组织中EMP 1蛋白的表达。采用非参数分析方法分析EMP 1表达与临床参数的相关性。结果RT-PCR和qRT-PCR结果显示，与正常口腔黏膜组织相比，口腔鳞癌组织中EMP 1的mRNA水平明显降低。免疫组化结果表明，EMP 1蛋白在口腔鳞癌中也下调（p=0.031）。EMP 1表达的降低与OSCC患者的临床分期（p=0.002）和淋巴结转移（p=0.044）显著相关。同时，在早期和晚期OSCC之间（p=0.003），以及在有淋巴结转移和无淋巴结转移的OSCC之间（p=0.045），分别存在显著差异。结论EMP 1可能是一种抑癌基因，与口腔鳞癌的淋巴结转移有关。
Aims To investigate the expression of EMP1 in oral squamous cell carcinoma (OSCC) tissues and its correlation with available clinical parameters of patients with OSCC. Methods The mRNA levels of EMP1 were measured in 18 paired OSCC and corresponding adjacent normal tissues using RT-PCR. Another 45 pairs of OSCC samples were selected to detect the mRNA level of EMP1 using quantitative RT-PCR (qRT-PCR). The protein levels of EMP1 were also evaluated in 60 cases of patients with OSCC using immunohistochemical staining. The correlation between EMP1 expression and clinical parameters was analysed with non-parametric analysis. Results The results of RT-PCR and qRT-PCR showed that, compared with the paired normal tissues, the mRNA levels of EMP1 were significantly decreased in OSCC. The immunohistochemical results indicated that the EMP1 protein was also downregulated in OSCC (p=0.031). Decreased expression of EMP1 was significantly correlated with clinical stage (p=0.002) and lymph node metastasis (p=0.044) of patients with OSCC. Meanwhile, there was a significant difference between OSCCs at early and advanced stages (p=0.003), and between OSCCs with lymph node metastasis and no lymph node metastasis (p=0.045), respectively. Conclusions The results suggest that EMP1 may be a tumour suppressor and associated with lymph node metastasis in OSCC.