Nanoclays mediate stem cell differentiation and mineralized ECM formation on biopolymer scaffolds
Nanoclays mediate stem cell differentiation and mineralized ECM formation on biopolymer scaffolds
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DOI：
10.1002/jbm.a.34561
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发表时间：
2013-09-01
影响因子：
4.9
通讯作者：
Katti, Kalpana S.
中科院分区：
文献类型：
--
作者：
Ambre, Avinash H.;Katti, Dinesh R.;Katti, Kalpana S.
在这项工作中，新型改性纳米粘土被用来矿化羟基磷灰石（HAP），模拟骨中的生物矿化。这种原位 HAPclay 被进一步掺入壳聚糖/聚半乳糖醛酸 (Chi/PgA) 支架和薄膜中，用于骨组织工程。根据使用 ChiPgA 生物聚合物系统原位 HAPclay 加工的变化，观察到支架微观结构的差异。使用成像和分析方法研究了人类间充质干细胞 (hMSC) 对这些支架和薄膜的反应。 SEM 显微照片表明 hMSC 能够粘附到 ChiPgA/原位 HAPclay 支架上，相差图像表明在没有用于 hMSC 分化的成骨补充剂的情况下，在 ChiPgA/原位 HAPclay 膜上形成矿化结节。通过茜素红 S 染料对结节的阳性染色证实 hMSC 形成矿化结节。活力和分化测定表明 ChiPgA/原位 HAPclay 支架有利于 hMSC 的活力和分化。进行了独特的两阶段细胞接种实验，作为增强 hMSC 在 ChiPgA/原位 HAPclay 复合膜上组织形成的策略。这项工作表明，基于 ChiPgA/原位 HAPclay 复合材料的生物材料可用于骨组织工程应用，原位纳米粘土-HAP 系统介导 hMSC 的骨诱导和骨传导反应。 (c) 2013 年 Wiley periodicals, Inc. J Biomed Mater Res Part A：101A：2644-2660，2013 年。
In this work, novel modified nanoclays were used to mineralize hydroxyapatite (HAP) mimicking biomineralization in bone. This in situ HAPclay was further incorporated into chitosan/polygalacturonic acid (Chi/PgA) scaffolds and films for bone tissue engineering. Differences in microstructure of the scaffolds were observed depending on the changes in processing of in situ HAPclay with ChiPgA biopolymer system. Response of human mesenchymal stem cells (hMSCs) on these scaffolds and films was studied using imaging and assays. SEM micrographs indicate that hMSCs were able to adhere to ChiPgA/in situ HAPclay scaffolds and phase contrast images indicated formation of mineralized nodules on ChiPgA/in situ HAPclay films in absence of osteogenic supplements used for differentiation of hMSCs. The formation of mineralized nodules by hMSCs was confirmed by positive staining of the nodules by Alizarin Red S dye. Viability and differentiation assays showed that ChiPgA/in situ HAPclay scaffolds were favorable for viability and differentiation of hMSCs. Unique two-stage cell seeding experiments were performed as a strategy to enhance tissue formation by hMSCs on ChiPgA/in situ HAPclay composite films. This work showed that biomaterials based on ChiPgA/in situ HAPclay composites can be used for bone tissue engineering applications and in situ nanoclay-HAP system mediates osteoinductive and osteoconductive response from hMSCs. (c) 2013 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 101A: 2644-2660, 2013.