Surgical and Oncological Factors Affecting the Successful Engraftment of Patient-derived Xenografts in Pancreatic Ductal Adenocarcinoma.
Surgical and Oncological Factors Affecting the Successful Engraftment of Patient-derived Xenografts in Pancreatic Ductal Adenocarcinoma.
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发表时间：
2016-02
影响因子：
2
通讯作者：
E. Jun;Jaeyoon Jung;Seong-Yun Jeong;E. Choi;M. Kim;Ji Sun Lee;Seung‐Mo Hong;H. Seol;Changmo Hwang;R. Hoffman;I. Shim;Suhwan Chang;Song-Cheol Kim
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作者：
E. Jun;Jaeyoon Jung;Seong-Yun Jeong;E. Choi;M. Kim;Ji Sun Lee;Seung‐Mo Hong;H. Seol;Changmo Hwang;R. Hoffman;I. Shim;Suhwan Chang;Song-Cheol Kim
为了在转化研究中有效地使用胰腺癌患者来源的异种移植物（PDX）模型，需要在免疫缺陷小鼠中成功地植入手术标本的PDX。材料与方法：共有102例患者接受了胰腺癌切除术，采用不同的程序。将所有患者的肿瘤组织皮下植入小鼠体内。测定小鼠中的肿瘤植入和生长。采用免疫组化法检测移植物与手术类型、时间、肿瘤大小和癌基因表达的相关性。结果多因素分析显示，患者肿瘤大小大于3.5cm是PDX植入成功的重要相关因素。与此相反，植入与手术操作、切除标本所需时间、肿瘤分化程度、淋巴结转移、p53、受体酪氨酸蛋白激酶erbB-2（CERBB2）或胰腺癌基因座4缺失（DPC4）蛋白表达无关。结论患者体内最小肿瘤体积是肿瘤植入成功的重要因素。
BACKGROUND To effectively use pancreatic cancer patient-derived xenograft (PDX) models in translational research, successful PDX engraftment of surgical specimens in immune-deficient mice is needed. MATERIALS AND METHODS A total of 102 patients underwent pancreatic cancer resection using various procedures. Tumor tissue from all patents was implanted subcutaneously into mice. Tumor engraftment and growth in mice were determined. Engraftment was tested for correlation with operation type, time, tumor size, and oncogene expression using immunohistoculture. RESULTS Multivariate analysis showed that a tumor size of more than 3.5 cm in the patient was a significant factor related to successful PDX engraftment. In contrast, there was no correlation of engraftment with surgical procedure, time needed to remove the specimen, tumor differentiation, lymph node metastasis, and protein expression of p53, Receptor tyrosine-protein kinase erbB-2 (CERBB2), or deleted in pancreatic carcinoma locus 4 (DPC4). CONCLUSION A minimum tumor size in the patient is an important factor for successful tumor engraftment.