Autorepression of RctB, an initiator of Vibrio cholerae chromosome II replication
Autorepression of RctB, an initiator of Vibrio cholerae chromosome II replication
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DOI：
10.1128/jb.188.2.789-793.2006
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发表时间：
2006-01-01
影响因子：
3.2
通讯作者：
Waldor, MK
中科院分区：
文献类型：
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作者：
Egan, ES;Duigou, S;Waldor, MK
RctB蛋白与霍乱弧菌染色体II（chrII）复制起点结合，是oriCII（Vc）复制所必需的。在这里，我们发现RctB作为一种自抑制因子，抑制RctB的转录。整合宿主因子促进rctB转录，而两个霍乱弧菌染色体复制所需的因子Dam和DnaA影响rctB的自抑制。因此，RctB似乎既作为启动物又作为转录抑制物来调节chrII的复制，并且它的合成受到控制两条染色体复制的因子的调节。
The RctB protein binds to the origin of replication of Vibrio cholerae chromosome II (chrII) and is required for oriCII(Vc)-based replication. Here, we found that RctB acts as an autorepressor, inhibiting rctB transcription. Integration host factor promotes rctB transcription, while Dam and DnaA, factors required for replication of both V cholerae chromosomes, influence RctB autorepression. Thus, RctB appears to regulate chrII replication as both an initiator and a transcription repressor, and its synthesis is modulated by factors that govern replication of both chromosomes.