Postweaning development of porcine small intestinal morphology and epithelial cell proliferation
Postweaning development of porcine small intestinal morphology and epithelial cell proliferation
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DOI：
10.2527/jas.2015-9774
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发表时间：
2016-09
影响因子：
3.3
通讯作者：
J. Kluess;S. Kahlert;J. Krüger;H. Rothkötter;A. Berk;S. Kersten;S. Dänicke
中科院分区：
文献类型：
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作者：
J. Kluess;S. Kahlert;J. Krüger;H. Rothkötter;A. Berk;S. Kersten;S. Dänicke
组织形态计量学和细胞增殖是肠道发育的标志，我们对猪从断奶到屠宰的 5 个不同小肠部位进行了研究。此外，研究了肠内脱氧雪腐镰刀菌烯醇（DON）暴露对肠道形态和增殖的影响。屠宰 4 个类别（断奶后 1、4、8 和 12 周）的断奶后猪（4-6 头/类别），并从十二指肠、近端空肠、中段空肠、近端和末端回肠收集肠壁。在组织学标本上测量绒毛高度和隐窝深度，并通过免疫组织化学（5'-溴-2'-脱氧尿苷阳性或磷酸组蛋白 H3 阳性细胞）测定隐窝区域的细胞增殖。所有猪都饲喂对照饮食或 DON 污染饮食（约 3 毫克/千克饲料），并分析肠轴、年龄和饮食影响的数据。绒毛高度仅受肠道部位、年龄或饮食的轻微影响，但隐窝深度和细胞增殖沿小肠轴显示出独特的模式。在猪中，无论年龄或饮食如何，十二指肠的隐窝最深（P < 0.05），但增殖细胞计数最低，尽管后者并不总是具有统计学意义。此外，年龄对十二指肠和上空肠水平的隐窝深度有很强的影响，但对下段肠道切片没有影响：断奶后1周（294μm）、4周（505μm）和8周（508μm）隐窝深度显着增加（P <0.01）。断奶后 4 周的动物和老年动物之间没有差异。我们无法确认隐窝深度和增殖细胞计数之间的正相关关系。饮食中 DON 的存在并没有显着改变小肠在绒毛高度、隐窝深度或上皮细胞增殖方面的发育。
Histomorphometry and cell proliferation are markers of intestinal development, which we investigated in pigs from weaning to slaughter at 5 different small intestinal sites. Furthermore, the impact of enteral deoxynivalenol (DON) exposure on intestinal morphology and proliferation was studied. Postweaning pigs of 4 categories (1, 4, 8, and 12 wk after weaning) were slaughtered (4–6 pigs/category) and intestinal wall was collected from the duodenum, proximal jejunum, mid jejunum, and proximal and terminal ileum. Villus height and crypt depths were measured on histological specimens, and cell proliferation was determined by immunohistochemistry (5′-bromo-2′-deoxyuridine–positive or phosphohistone H3–positive cells) in the crypt area. All pigs were fed either a control diet or a DON-contaminated diet (approximately 3 mg/kg feed), and data were analyzed for the effect of gut axis, age, and diet. Villus height was only marginally affected by intestinal site, age, or diet, but crypt depth and cell proliferation showed a distinct pattern along the small intestinal axis. In pigs, irrespective of age or diet, the duodenum exhibited the deepest crypts (P < 0.05) but the lowest count of proliferating cell, albeit the latter was not always statistically significant. Moreover, there was a strong age effect on crypt depth at the duodenal and upper jejunal level but not in the lower intestinal sections: crypt depth increased dramatically (P < 0.01) between 1 (294 μm), 4 (505 μm), and 8 wk after weaning (508 μm). There was no difference between the 4-wk-after-weaning animals and older animals. We could not confirm a positive relationship between crypt depth and proliferating cell count. The presence of dietary DON did not significantly modify the development of the small intestine with respect to villus height, crypt depth, or epithelial cell proliferation.