Inheritance of L1014F and M918T sodium channel mutations associated with pyrethroid resistance in Myzus persicae
Inheritance of L1014F and M918T sodium channel mutations associated with pyrethroid resistance in Myzus persicae
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DOI：
10.1098/rsbl.2008.0225
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发表时间：
2008-10-23
期刊：
影响因子：
3.3
通讯作者：
Denholm, Ian
中科院分区：
文献类型：
--
作者：
Eleftherianos, Ioannis;Foster, Stephen P.;Denholm, Ian
电压门控钠通道中的两个氨基酸取代（L1014F 和 M918T）赋予桃马铃薯蚜 Myzus persicae 靶位点对拟除虫菊酯杀虫剂的抗性。在实验室条件下对具有各种突变组合的拟除虫菊酯抗性和敏感桃蚜克隆进行杂交，并通过钠通道基因的 IIS4-S6 区域的直接 DNA 测序来分析蚜虫后代的基因型。分离模式表明，在 L1014F 和 M918T 杂合的蚜虫中，两种突变都存在于相同的抗性等位基因中。尽管这些突变在其他害虫物种中主要表现为隐性，但这些蚜虫在浸叶生物测定中表现出对拟除虫菊酯的强烈抗性。这些结果对于野外种群中拟除虫菊酯抗性的传播和管理具有重要意义。
Two amino acid substitutions (L1014F and M918T) in the voltage-gated sodium channel confer target-site resistance to pyrethroid insecticides in the peach potato aphid, Myzus persicae. Pyrethroid-resistant and -susceptible M. persicae clones with various combinations of these mutations were crossed under laboratory conditions, and the genotypes of aphid progeny were analysed by direct DNA sequencing of the IIS4-S6 region of the sodium channel gene. Segregation patterns showed that in aphids heterozygous for both L1014F and M918T, both mutations were present in the same resistance allele. Despite these mutations appearing largely recessive in other pest species, such aphids exhibited strong resistance to pyrethroids in leaf-dip bioassays. These results have important implications for the spread and management of pyrethroid resistance in field populations.