The toxicity mechanism of sodium fluoride on fertility in female rats.
The toxicity mechanism of sodium fluoride on fertility in female rats.
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DOI：
10.1016/j.fct.2013.09.023
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发表时间：
2013-12
期刊：
影响因子：
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通讯作者：
Yongjiang Zhou;Yiwen Qiu;Junlin He;Xuemei Chen;Yubin Ding;Yingxiong Wang;Xue-qing Liu
中科院分区：
文献类型：
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作者：
Yongjiang Zhou;Yiwen Qiu;Junlin He;Xuemei Chen;Yubin Ding;Yingxiong Wang;Xue-qing Liu
人们越来越认识到氟化钠（NaF）对人类生殖的有害影响，特别是与女性生殖有关的影响。然而，NaF干扰雌性生殖的机制尚不清楚。本研究的目的是调查氟暴露对女性生育力的影响，并阐明这些影响的机制。将雌性Sprague-Dawley大鼠分为3组：1个对照组和2个氟化钠处理组（100和200 mg/L饮用水，持续12周）。评价了几个参数，包括：（i）氟化物浓度;（ii）雌激素（E2）和孕激素（P）浓度;（iii）雌激素受体α蛋白（ERα）;（iv）孕激素受体（PgR）蛋白;（v）促卵泡激素受体（FSHR）和促黄体激素受体（LHR）蛋白。结果表明，NaF可显著降低血清E2和P水平，并降低FSHR蛋白表达。另外，氟暴露显著增加了Erα和PgR蛋白表达水平以及LHR蛋白表达水平。这些结果表明，生殖激素的减少和相关受体蛋白表达的异常是在雌性大鼠中观察到的生育能力下降的重要因素，暴露于氟化钠。
Recognition of the harmful effects of sodium fluoride (NaF) on human reproduction is increasing, especially as it relates to female reproduction. However, the mechanism by which NaF interferes with female reproduction is unclear. The aims of the present study were to investigate the effects of fluoride exposure on female fertility and to elucidate the mechanisms underlying these effects. Female Sprague–Dawley rats were divided into three groups: one control group and two NaF-treated groups (100 and 200 mg/L in the drinking water for 12 weeks). Several parameters were evaluated, including: (i) fluoride concentrations; (ii) estrogen (E2) and progesterone (P) concentrations; (iii) estrogen receptor alpha protein (ERα); (iv) progesterone receptor (PgR) protein; (v) follicle-stimulating hormone receptor (FSHR) and luteinizing hormone receptor (LHR) protein. The results indicated that administration of NaF lead to significant decreases in E2and P levels in the serum and in the expression of FSHR protein. In addition, fluoride exposure significantly increased Erα and PgR protein expression levels and LHR protein expression. These results suggest that the reproductive hormone reduction and the abnormalities of related receptor proteins expression are important factors underlying the decreased fertility observed in female rats that have been exposed to NaF.