Transcriptomic Characterization of Temperature Stress Responses in Larval Zebrafish
Transcriptomic Characterization of Temperature Stress Responses in Larval Zebrafish
复制标题
斑马鱼幼虫温度应激反应的转录组特征
DOI：
10.1371/journal.pone.0037209
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发表时间：
2012-05-30
期刊：
影响因子：
3.7
通讯作者：
Cui, Zongbin
中科院分区：
文献类型：
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作者：
Long, Yong;Li, Linchun;Cui, Zongbin
温度影响鱼类几乎所有的生化、生理和生活史活动，但温度驯化的分子机制仍不清楚。以往的研究已经在成鱼组织中发现了许多温度调控基因，然而，对温度胁迫下仔鱼的转录反应还没有很好的了解。在这项研究中，我们的特点是在斑马鱼幼虫暴露于冷或热应激基因芯片分析的转录反应。与28°C对照相比，96只hpf幼虫在冷（16°C）或热（34°C）处理2和48 h后，共有2680个基因受到影响，其中大部分基因的表达具有温度特异性和时间调控性。生物信息学分析确定了多种温度调节的生物过程和途径。温度胁迫诱导的生物学过程包括转录调控、核小体组装、染色质组织和蛋白质折叠等。然而，RNA加工、细胞金属离子稳态和蛋白质转运等过程在冷暴露48 h下表达上调。途径，如mTOR信号，p53信号和昼夜节律之间的冷诱导的基因，而脂肪细胞因子信号，蛋白质输出和精氨酸和脯氨酸代谢之间的热诱导的基因富集。虽然这些生物学过程和途径大多受冷或热的特异性调控，但也发现了鱼类对冷和热胁迫的共同反应，因此，这些发现为阐明鱼类温度驯化的机制提供了新的有趣线索。
Temperature influences nearly all biochemical, physiological and life history activities of fish, but the molecular mechanisms underlying the temperature acclimation remains largely unknown. Previous studies have identified many temperature-regulated genes in adult tissues; however, the transcriptional responses of fish larvae to temperature stress are not well understood. In this study, we characterized the transcriptional responses in larval zebrafish exposed to cold or heat stress using microarray analysis. In comparison with genes expressed in the control at 28°C, a total of 2680 genes were found to be affected in 96 hpf larvae exposed to cold (16°C) or heat (34°C) for 2 and 48h and most of these genes were expressed in a temperature-specific and temporally regulated manner. Bioinformatic analysis identified multiple temperature-regulated biological processes and pathways. Biological processes overrepresented among the earliest genes induced by temperature stress include regulation of transcription, nucleosome assembly, chromatin organization and protein folding. However, processes such as RNA processing, cellular metal ion homeostasis and protein transport and were enriched in genes up-regulated under cold exposure for 48 h. Pathways such as mTOR signalling, p53 signalling and circadian rhythm were enriched among cold-induced genes, while adipocytokine signalling, protein export and arginine and praline metabolism were enriched among heat-induced genes. Although most of these biological processes and pathways were specifically regulated by cold or heat, common responses to both cold and heat stresses were also found. Thus, these findings provide new interesting clues for elucidation of mechanisms underlying the temperature acclimation in fish.