Coated protein nanoclusters from influenza H7N9 HA are highly immunogenic and induce robust protective immunity.
Coated protein nanoclusters from influenza H7N9 HA are highly immunogenic and induce robust protective immunity.
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DOI：
10.1016/j.nano.2016.09.001
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发表时间：
2017-01
期刊：
影响因子：
--
通讯作者：
Wang BZ
中科院分区：
文献类型：
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作者：
Wang L;Chang TZ;He Y;Kim JR;Wang S;Mohan T;Berman Z;Tompkins SM;Tripp RA;Compans RW;Champion JA;Wang BZ
反复出现的流感病毒每年都会对公众健康构成威胁。一种节省时间、成本效益高和不依赖鸡蛋的流感疫苗方法非常重要，尤其是在应对新出现的大流行时。我们从禽源H7N9甲型流感病毒的重组三聚体血凝素中制备了包被的双层蛋白质纳米簇，作为一种应对新出现的大流行的疫苗开发方法。对用纳米簇免疫的小鼠的病毒特异性免疫反应和保护效果的评估表明，候选疫苗具有高度的免疫原性，能够在不使用佐剂的情况下诱导对该病毒的保护性免疫和持久的体液抗体反应。由于高度免疫原性涂层纳米簇的优势还包括在不依赖鸡蛋的系统中快速生产，这种方法不仅在应对新出现的大流行方面具有巨大的潜力，而且作为传统季节性流感疫苗的替代品具有巨大的潜力。以H7N9流感病毒重组三聚体HA蛋白为原料，通过去溶、交联等方法制备包被蛋白纳米簇。由此产生的纳米簇具有高度的免疫原性，能够诱导高水平的抗体反应，从而对小鼠的活病毒攻击提供免疫保护。
Recurring influenza viruses pose an annual threat to public health. A time-saving, cost-effective and egg-independent influenza vaccine approach is important particularly when responding to an emerging pandemic. We fabricated coated, two-layer protein nanoclusters from recombinant trimeric hemagglutinin from an avian-origin H7N9 influenza A virus as an approach for vaccine development in response to an emerging pandemic. Assessment of the virus-specific immune responses and protective efficacy in mice immunized with the nanoclusters demonstrated that the vaccine candidates were highly immunogenic, able to induce protective immunity and long-lasting humoral antibody responses to this virus without the use of adjuvants. Because the advantages of the highly immunogenic coated nanoclusters also include rapid productions in an egg-independent system, this approach has great potential for influenza vaccine production not only in response to an emerging pandemic, but also as a replacement for conventional seasonal influenza vaccines. Coated protein nanoclusters were fabricated from recombinant trimeric HA protein of H7N9 influenza virus by desolvation followed by crosslinking. The resulting nanoclusters were highly immunogenic, able to induce high levels of antibody responses conferring immune protection against live virus challenge in mice.