Evidence for a persistent microbial seed bank throughout the global ocean
Evidence for a persistent microbial seed bank throughout the global ocean
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DOI：
10.1073/pnas.1217767110
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发表时间：
2013-03-19
影响因子：
11.1
通讯作者：
Gilbert, Jack A.
中科院分区：
文献类型：
--
作者：
Gibbons, Sean M.;Caporaso, J. Gregory;Gilbert, Jack A.
细菌分类群是否像大型生物一样表现出明显的特有性，或者一个地点的细菌群落能否重新获得世界海洋的总系统发育多样性？在这里，我们比较了来自英吉利海峡一个地点（L4-DeepSeq）的深层细菌群落特征与来自地球仪的国际海洋微生物普查（ICoMM）的356个数据集（从海洋浮游层和沉积物样本到海绵相关环境）。在L4-DeepSeq站点，增加测序深度揭示了与全球ICoMM数据更大的系统发育重叠。该网站包含31.7-66.2%的操作分类单位在一个给定的ICoMM生物群系中确定。这种重叠的外推表明，来自L4位点的1.93 × 10（11）序列将捕获所有ICoMM细菌系统发育多样性。目前的技术趋势表明，这一限制可能在3年内达到。这些结果强烈表明，海洋生物圈保持着以前未被发现的持久性微生物种子库。
Do bacterial taxa demonstrate clear endemism, like macroorganisms, or can one site's bacterial community recapture the total phylogenetic diversity of the world's oceans? Here we compare a deep bacterial community characterization from one site in the English Channel (L4-DeepSeq) with 356 datasets from the International Census of Marine Microbes (ICoMM) taken from around the globe (ranging from marine pelagic and sediment samples to sponge-associated environments). At the L4-DeepSeq site, increasing sequencing depth uncovers greater phylogenetic overlap with the global ICoMM data. This site contained 31.7-66.2% of operational taxonomic units identified in a given ICoMM biome. Extrapolation of this overlap suggests that 1.93 x 10(11) sequences from the L4 site would capture all ICoMM bacterial phylogenetic diversity. Current technology trends suggest this limit may be attainable within 3 y. These results strongly suggest the marine biosphere maintains a previously undetected, persistent microbial seed bank.