Characterization of 26 new microsatellite loci in the dugong (Dugong dugon)

Characterization of 26 new microsatellite loci in the dugong (Dugong dugon)
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DOI:
10.1111/j.1471-8286.2007.01853.x
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发表时间:
2007-11-01
期刊:
MOLECULAR ECOLOGY NOTES
影响因子:
--
通讯作者:
Lanyon, Janet M.
Lanyon, Janet M.
中科院分区:
其他
文献类型:
--
作者:
Broderick, Damien;Ovenden, Jenny;Lanyon, Janet M.

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从儒艮(Dugong dugon)中分离到26个微卫星位点。平均杂合度为0.52,每个位点有2 ~ 10个等位基因。这些标记适用于儒艮的遗传标记重捕获(P-ID = 5 × 10 ~(-16)),也可用于物理标记丢失的量化、亲缘关系的估计、亲子鉴定、种群结构的阐明和迁徙者的识别。在佛罗里达海牛(22/26)和亚洲象(6/26)中也有扩增。
Twenty-six microsatellite loci have been isolated from a dugong (Dugong dugon). The average heterozygosity was 0.52 with two to 10 alleles per locus surveyed from 50 individuals. The markers are suitable for genetic mark-recapture (P-ID = 5 x 10(-16)) in dugongs and they could also be used to quantify physical tag loss, estimate relatedness, assign paternity, elucidate population structure and identify migrants. The loci also amplified in Florida manatees (22/26) and Asian elephants (6/26).