Nanopore sensor for fast label-free detection of short double-stranded DNAs
Nanopore sensor for fast label-free detection of short double-stranded DNAs
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DOI：
10.1016/j.bios.2006.12.003
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发表时间：
2007-06-15
影响因子：
12.6
通讯作者：
Ko, Christopher
中科院分区：
文献类型：
--
作者：
Kim, Young-Rok;Min, Junhong;Ko, Christopher
功能化表面通过增加短双链 DNA 的易位持续时间来增强制造的纳米孔的分子传感能力。纳米孔的表面用-γ-苯氨基丙基三乙氧基硅烷衍生化，当DNA分子位于纳米孔附近时，带正电荷的表面会吸引DNA分子。由于强静电相互作用，DNA 的易位持续时间增加，这使我们能够检测到低于传统固态纳米孔传感器尺寸限制的短双链 DNA (< 1 kbp)。使用表面功能化纳米孔分析了 539 bp 和 910 bp 双链 DNA，并在这项工作中介绍了它们的易位动力学。表面修饰纳米孔的新功能可以检测短双链 DNA 分子，可以很容易地应用于芯片实验室型 DNA 传感器中的快速无标记诊断分析。 (c) 2006 Elsevier B.V. 保留所有权利。
Functionalizing surface enhanced the molecular sensing ability of a fabricated nanopore by increasing the translocation duration time for a short double-stranded DNA. The surface of nanopore was derivatized with -y-aniinopropyltriethoxysilane and the positively charged surface attracted DNA molecules when they were in the vicinity of nanopore. The translocation duration time of DNA increased due to the strong electrostatic interaction and it enabled us to detect a short double-stranded DNA (< 1 kbp) that is under the size limit of a conventional solid state nanopore sensor. Both 539 and 910 bp double-stranded DNAs were analyzed with the surface functionalized nanopore and their translocation kinetics are presented in this work. The new feature of the surface modified nanopore that can detect short double-stranded DNA molecules could readily be applied for a rapid label-free diagnostic analysis in a Lab-On-a-Chip type DNA sensor. (c) 2006 Elsevier B.V. All rights reserved.