Nuclear transport of baculovirus: Revealing the nuclear pore complex passage
Nuclear transport of baculovirus: Revealing the nuclear pore complex passage
复制标题
DOI：
10.1016/j.jsb.2011.11.006
复制
发表时间：
2012-01-01
影响因子：
3
通讯作者：
Pante, Nelly
中科院分区：
文献类型：
--
作者：
Au, Shelly;Pante, Nelly
杆状病毒是在其宿主细胞的细胞核中复制的最大病毒之一。在感染过程中，含有DNA病毒基因组的衣壳被释放到细胞质中，并通过一种基本上未知的机制将基因组递送到细胞核中。在这里，我们使用了杆状病毒苜蓿银纹夜蛾多核型多角体病毒的衣壳与电子显微镜相结合，发现这种衣壳穿过NPC并完整地进入细胞核，在那里它释放出它的基因组。为了更好地说明这种衣壳的存在，通过NPC在其天然构象，我们重建核输入事件使用电子断层扫描。此外，使用不同的实验条件，我们能够可视化完整的衣壳与NPC细胞质丝相互作用，作为初始对接位点，并中途通过NPC。我们的数据表明，NPC中央通道经历了大规模的重排，使完整的250 nm长的杆状病毒衣壳易位。我们讨论了我们的结果在NPC功能的假设模型。(C)2011 Elsevier Inc. All rights reserved.
Baculoviruses are one of the largest viruses that replicate in the nucleus of their host cells. During an infection the capsid, containing the DNA viral genome, is released into the cytoplasm and delivers the genome into the nucleus by a mechanism that is largely unknown. Here, we used capsids of the baculovirus Autographa californica multiple nucleopolyhedrovirus in combination with electron microscopy and discovered this capsid crosses the NPC and enters into the nucleus intact, where it releases its genome. To better illustrate the existence of this capsid through the NPC in its native conformation, we reconstructed the nuclear import event using electron tomography. In addition, using different experimental conditions, we were able to visualize the intact capsid interacting with NPC cytoplasmic filaments, as an initial docking site, and midway through the NPC. Our data suggests the NPC central channel undergoes large-scale rearrangements to allow translocation of the intact 250-nm long baculovirus capsid. We discuss our results in the light of the hypothetical models of NPC function. (C) 2011 Elsevier Inc. All rights reserved.