Derivation of Thyroid Follicular Cells From Pluripotent Stem Cells: Insights From Development and Implications for Regenerative Medicine.
Derivation of Thyroid Follicular Cells From Pluripotent Stem Cells: Insights From Development and Implications for Regenerative Medicine.
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DOI：
10.3389/fendo.2021.666565
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发表时间：
2021
影响因子：
5.2
通讯作者：
Kotton DN
中科院分区：
文献类型：
--
作者：
Posabella A;Alber AB;Undeutsch HJ;Droeser RA;Hollenberg AN;Ikonomou L;Kotton DN
以干细胞为基础的重建体内器官功能的疗法在未来的各种疾病的临床应用中有着巨大的前景。由于先天性缺乏功能性甲状腺细胞、外科手术切除组织或腺体切除而导致的甲状腺功能减退是一个特别有吸引力的内分泌疾病靶点，只要能够产生自体细胞并克服各种技术和生物学挑战，可以通过移植长期存活的功能性甲状腺前体细胞或成熟的滤泡上皮细胞来治愈。在这里，我们回顾了新出现的文献，表明甲状腺滤泡上皮细胞现在可以从小鼠、正常人或先天性甲状腺功能减退症患者的多潜能干细胞(PSCs)体外培养出来。我们回顾了甲状腺在体内的胚胎发育，并解释了发育生物学中的新发现如何被用作在体外将类似早期胚胎细胞的PSCs驱动成成熟的功能甲状腺滤泡的路线图。最后，我们讨论了现在需要解决的生物工程、生物学和临床障碍，如果要通过基于细胞和/或基因的治疗来实现终生治愈甲状腺功能减退症的目标。
Stem cell-based therapies to reconstitute in vivo organ function hold great promise for future clinical applications to a variety of diseases. Hypothyroidism resulting from congenital lack of functional thyrocytes, surgical tissue removal, or gland ablation, represents a particularly attractive endocrine disease target that may be conceivably cured by transplantation of long-lived functional thyroid progenitors or mature follicular epithelial cells, provided a source of autologous cells can be generated and a variety of technical and biological challenges can be surmounted. Here we review the emerging literature indicating that thyroid follicular epithelial cells can now be engineered in vitro from the pluripotent stem cells (PSCs) of mice, normal humans, or patients with congenital hypothyroidism. We review the in vivo embryonic development of the thyroid gland and explain how emerging discoveries in developmental biology have been utilized as a roadmap for driving PSCs, which resemble cells of the early embryo, into mature functional thyroid follicles in vitro. Finally, we discuss the bioengineering, biological, and clinical hurdles that now need to be addressed if the goals of life-long cure of hypothyroidism through cell- and/or gene-based therapies are to be attained.
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影响因子：
14.8
作者：
Dai HQ;Liang Z;Chang AN;Chapdelaine-Williams AM;Alvarado B;Pollen AA;Alt FW;Schwer B
通讯作者：
Schwer B
影响因子：
23.9
作者：
Bondue, Antoine;Lapouge, Gaelle;Blanpain, Cedric
通讯作者：
Blanpain, Cedric
影响因子：
4.8
作者：
Arufe, Maria C.;Lu, Min;Lin, Reigh-Yi
通讯作者：
Lin, Reigh-Yi
影响因子：
2.5
作者：
Fagman, H;Andersson, L;Nilsson, M
通讯作者：
Nilsson, M
DOI：
10.1016/j.bbrc.2009.02.035
发表时间：
2009-04-03
影响因子：
3.1
作者：
Arufe, Maria C.;Lu, Min;Lin, Reigh-Yi
通讯作者：
Lin, Reigh-Yi