A novel envelope protein involved in White spot syndrome virus infection
A novel envelope protein involved in White spot syndrome virus infection
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DOI：
10.1099/vir.0.80923-0
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发表时间：
2005-05-01
影响因子：
3.8
通讯作者：
Shi, ZL
中科院分区：
文献类型：
--
作者：
Huang, R;Xie, YL;Shi, ZL
来自白斑综合征病毒（WSSV）基因组的一个开放阅读框（命名为vp76）具有细胞因子I受体的基序，并已被鉴定为结构蛋白。本文通过在大肠杆菌中表达vp76，制备特异性抗体，确定相应蛋白在完整病毒粒子、核衣壳和WSSV包膜中的位置。VP76抗血清Western blotting证实VP76为WSSV包膜蛋白。为了研究VP76的功能，用不同浓度的VP76特异性抗血清中和WSSV，并肌肉注射到小龙虾体内。死亡率曲线显示，VP76抗血清能部分减弱WSSV感染，提示VP76是参与WSSV感染的包膜蛋白。
One open reading frame (designated vp76) from the White spot syndrome virus (WSSV) genome has the motif of a cytokine I receptor and has been identified as a structural protein. In this paper, vp76 was expressed in Escherichia coli and used to prepare a specific antibody to determine the location of the corresponding protein in the intact virion, the nucleocapsids and the envelope of WSSV. Western blotting with the VP76 antiserum confirmed that VP76 was an envelope protein of WSSV. To investigate the function of the VP76, WSSV was neutralized with the VP76-specific antiserum at different concentrations and injected intramuscularly into crayfish. The mortality curves showed that the VP76 antiserum could partially attenuate infection with WSSV, suggesting that VP76 is an envelope protein involved in WSSV infection.