Dental pulp stem cells senescence and regenerative potential relationship
Dental pulp stem cells senescence and regenerative potential relationship
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DOI：
10.1002/jcp.27472
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发表时间：
2019-05-01
影响因子：
5.6
通讯作者：
Belmonte, Monica Mattioli
中科院分区：
文献类型：
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作者：
Iezzi, Iolanda;Cerqueni, Giorgia;Belmonte, Monica Mattioli
牙髓炎和牙周炎的简单治疗仍然具有挑战性，再生方法可以满足这种偶然性。牙髓干细胞（DPSCs）是口腔康复治疗的良好候选者。在这里，我们研究了从不同年龄的供体中获取的人DPSCs的形态、增殖和向间充质和神经元表型的体外分化的变化。衰老是一种随着时间的推移而发生的生理现象，它阻碍了身体维持体内平衡的能力，也影响了功能储备。通过细胞荧光法、免疫组织化学、定量逆转录聚合酶链反应（qRT-PCR）和western blot分析，深入了解老年人成功的再生策略。我们观察到随着年龄的增长，DPSCs的增殖和分化潜力下降。有趣的是，这些细胞在成骨或牙源性刺激下表现不同，表现出不同的与年龄相关的矿化能力。同样，神经源性分化随着年龄的增长而减少。总之，我们的观察结果为在老龄化社会中制定量身定制的再生策略提供了有效的工具。
Uncomplicated treatments for pulpitis and periodontitis continues to be challenging and regenerative approaches could meet this contingency. Dental pulp stem cells (DPSCs) represent a good candidate for oral recovering therapies. Here, we investigated changes in morphology, proliferation, and in vitro differentiation toward mesenchymal and neuronal phenotypes of human DPSCs harvested from differently aged donors. Aging is a physiologic phenomenon occurring with time that hamper body's capability to maintain homeostasis also affecting the functional reserve. Cytofluorimetric, immunohistochemical, quantitative reverse-transcription polymerase chain reaction (qRT-PCR), and western blot analyses were performed to gain insight for successful regenerative strategies in elderly. We observed a decline in DPSCs proliferation and differentiation potential with age. Interestingly, these cells behaved differently under osteogenic or odontogenic stimuli, showing different age-related mineralization capabilities. Similarly, neurogenic differentiation decreased with age. In conclusion, our observations represent a valid tool for the development of tailored regenerative strategies in an aging society.