Muscle regeneration by reconstitution with bone marrow or fetal liver cells from green fluorescent protein-gene transgenic mice.
Muscle regeneration by reconstitution with bone marrow or fetal liver cells from green fluorescent protein-gene transgenic mice.
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发表时间：
2002-03
影响因子：
4
通讯作者：
S. Fukada;Y. Miyagoe‐Suzuki;H. Tsukihara;K. Yuasa;S. Higuchi;S. Ono;K. Tsujikawa;S. Takeda;Hiroshi Yamamoto
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文献类型：
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作者：
S. Fukada;Y. Miyagoe‐Suzuki;H. Tsukihara;K. Yuasa;S. Higuchi;S. Ono;K. Tsujikawa;S. Takeda;Hiroshi Yamamoto
骨髓和胎肝细胞的成肌潜能使用来自绿色荧光蛋白（GFP）基因转基因小鼠的供体细胞转移到嵌合体小鼠中进行检查。接受转基因小鼠骨髓细胞的X染色体连锁肌营养不良症（mdx）小鼠接受致死性辐照后，表现出大量的荧光（+）和肌营养不良蛋白（+）肌纤维。为了比较新生嵌合体中胎肝细胞和骨髓细胞的生成能力，将来自转基因小鼠的这两种细胞类型注射到白消安处理的正常或mdx新生小鼠中，并检查嵌合体中的肌肉生成。在嵌合体中，肉毒毒素诱导的（或对于mdx受体，未诱导的）肌肉再生也产生荧光（+）肌纤维。骨髓细胞的肌肉重建效率与胎肝细胞相当。然而，生肌细胞的频率高于胎儿肝脏比骨髓。在正常受体的新生儿嵌合体中，一些纤维在心脏毒素未处理的肌肉中表达荧光。此外，在嵌合体心脏毒素未处理肌肉的基底层下方观察到荧光（+）单核细胞，这是卫星细胞定位的位置。在未处理的新生嵌合体肌肉的增殖培养物中观察到单核荧光（+）和结蛋白（+）细胞。在接受来自心脏毒素未处理的新生嵌合体的肌肉单细胞的第二受体小鼠中产生荧光（+）肌纤维。结果表明，骨髓和胎肝细胞可能有潜力分化成肌肉卫星细胞，并参与肌肉损伤后的肌肉再生以及生理肌肉生成。
The myogenic potential of bone marrow and fetal liver cells was examined using donor cells from green fluorescent protein (GFP)-gene transgenic mice transferred into chimeric mice. Lethally irradiated X-chromosome-linked muscular dystrophy (mdx) mice receiving bone marrow cells from the transgenic mice exhibited significant numbers of fluorescence(+) and dystrophin(+) muscle fibres. In order to compare the generating capacity of fetal liver cells with bone marrow cells in neonatal chimeras, these two cell types from the transgenic mice were injected into busulfantreated normal or mdx neonatal mice, and muscular generation in the chimeras was examined. Cardiotoxin-induced (or -uninduced, for mdx recipients) muscle regeneration in chimeras also produced fluorescence(+) muscle fibres. The muscle reconstitution efficiency of the bone marrow cells was almost equal to that of fetal liver cells. However, the myogenic cell frequency was higher in fetal livers than in bone marrow. Among the neonatal chimeras of normal recipients, several fibres expressed the fluorescence in the cardiotoxin-untreated muscle. Moreover, fluorescence(+) mononuclear cells were observed beneath the basal lamina of the cardiotoxin-untreated muscle of chimeras, a position where satellite cells are localizing. It was also found that mononuclear fluorescence(+) and desmin(+) cells were observed in the explantation cultures of untreated muscles of neonatal chimeras. The fluorescence(+) muscle fibres were generated in the second recipient mice receiving muscle single cells from the cardiotoxin-untreated neonatal chimeras. The results suggest that both bone marrow and fetal liver cells may have the potential to differentiate into muscle satellite cells and participate in muscle regeneration after muscle damage as well as in physiological muscle generation.