AIR-FILLED PROTEINACEOUS MICROBUBBLES - SYNTHESIS OF AN ECHO-CONTRAST AGENT
AIR-FILLED PROTEINACEOUS MICROBUBBLES - SYNTHESIS OF AN ECHO-CONTRAST AGENT
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DOI：
10.1073/pnas.88.17.7708
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发表时间：
1991-09-01
影响因子：
11.1
通讯作者：
SUSLICK, KS
中科院分区：
文献类型：
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作者：
GRINSTAFF, MW;SUSLICK, KS
充气微泡在临床上用作超声应用的回声造影剂。空气填充的蛋白质微泡的水悬浮液的合成涉及在O2存在下的蛋白质水溶液的超声辐照。人和牛血清白蛋白微泡的产量和尺寸分布已被确定为各种实验参数的函数。这些微泡的化学性质和它们显着的长寿命的起源进行了探索。微泡主要通过半胱氨酸残基的蛋白质间交联保持在一起。主要的交联剂是超氧化物，由声空化过程中产生的极高温度产生。
Air-filled microbubbles are in clinical use as echo-contrast agents for sonographic applications. The synthesis of aqueous suspensions of air-filled proteinaceous microbubbles involves the ultrasonic irradiation of aqueous protein solutions in the presence of O2. Yields and size distributions of human and bovine serum albumin microbubbles have been determined as a function of various experimental parameters. The chemical nature of these microbubbles and the origin of their remarkably long lifetimes have been explored. The microbubbles are held together primarily by interprotein cross-linking of cysteine residues. The principal cross-linking agent is superoxide created by the extremely high temperatures produced during acoustic cavitation.