Autoimmune Hepatitis: Shifts in Gut Microbiota and Metabolic Pathways among Egyptian Patients
Autoimmune Hepatitis: Shifts in Gut Microbiota and Metabolic Pathways among Egyptian Patients
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DOI：
10.3390/microorganisms8071011
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发表时间：
2020-07-01
期刊：
影响因子：
4.5
通讯作者：
Hetta, Helal F.
中科院分区：
文献类型：
--
作者：
Elsherbiny, Nahla M.;Rammadan, Mohammed;Hetta, Helal F.
自身免疫性肝炎（AIH）是一种具有复杂免疫发病机制的慢性炎症性疾病。生态失调与许多自身免疫性疾病有关，但其在自身免疫性肝炎（AIH）中的详细作用仍需要严格评估，特别是在埃及。我们的目的是确定与健康个体相比，AIH埃及患者肠道微生物群特征和由此产生的代谢途径的变化。从15名AIH初治患者和10名健康个体中收集粪便样本。利用Illumina MiSeq平台对16 S rRNA基因V3-V4高变区进行扩增和测序。AIH患者的细菌多样性显著低于对照组。门水平的分析表明，厚壁菌门，拟杆菌和变形菌的代表性过高。在属的水平上，与AIH相关的粪杆菌属、布劳特氏菌属、链球菌属、嗜血杆菌属、拟杆菌属、韦荣氏球菌属、真杆菌属、毛螺菌属和丁酸球菌属的富集与普雷沃氏菌属、副拟杆菌属和扩张杆菌属的富集相反，在这些患者中，这些菌显著减少。总的来说，AIH患者中与生态失调相关的预测代谢途径可以协调肠道微生物群在自身免疫性疾病中的潜在致病作用，尽管不是以疾病特异性的方式，需要未来的大规模研究。
Autoimmune hepatitis (AIH) is a chronic inflammatory disorder with complex immunopathogenesis. Dysbiosis has been linked to many autoimmune diseases, but its detailed role in autoimmune hepatitis (AIH) still needs rigorous evaluation, especially in Egypt. We aimed to identify the shift in the gut microbiota profile and resultant metabolic pathways in AIH Egyptian patients compared to healthy individuals. Stool samples were collected from 15 AIH-naive patients and from 10 healthy individuals. The V3-V4 hyper-variable regions in16S rRNA gene was amplified and sequenced using Illumina MiSeq platform. Significantly lower bacterial diversity in AIH patients was found compared to the controls. A phylum-level analysis showed the overrepresentation of Firmicutes, Bacteroides, and Proteobacteria. At the genus level, AIH-associated enrichment ofFaecalibacterium,Blautia,Streptococcus,Haemophilus, Bacteroides,Veillonella,Eubacterium,LachnospiraceaeandButyricicoccuswas reported in contrast toPrevotella,ParabacteroidesandDilaster, which were significantly retracted in such patients. Overall, the predicted metabolic pathways associated with dysbiosis in AIH patients could orchestrate the potential pathogenic roles of gut microbiota in autoimmune disease, though not in a disease-specific manner, calling for future large-scale studies.