Apolipoprotein mRNA in liver and intestine of rats is affected by dietary beet fiber or cholestyramine.
Apolipoprotein mRNA in liver and intestine of rats is affected by dietary beet fiber or cholestyramine.
复制标题
大鼠肝脏和肠道中的载脂蛋白 mRNA 受到膳食甜菜纤维或考来烯胺的影响。
DOI：
10.1093/jn/125.1.13
复制
发表时间：
1995
期刊：
影响因子：
--
通讯作者：
R. Niki
中科院分区：
文献类型：
--
作者：
K. Sonoyama;H. Nishikawa;S. Kiriyama;R. Niki
给大鼠喂食无胆固醇饮食，不添加纤维（无纤维）或添加15 g/100 g甜菜纤维或5 g/100 g考来烯胺14 d。最终血浆总胆固醇浓度显着低于大鼠饲料甜菜纤维比那些美联储纤维或消胆胺饮食。这种差异主要是由于HDL胆固醇浓度较低。甜菜纤维组血浆载脂蛋白A-I浓度也有降低的趋势（P < 0.1）。北方印迹分析表明，空肠载脂蛋白A-I和A-IV mRNA的相对浓度在所有组中是相同的，而回肠载脂蛋白A-I和A-IV mRNA水平显着低于饲喂甜菜纤维或消胆胺的大鼠比那些在无纤维饮食。肝脏载脂蛋白E mRNA的浓度在所有组中是相同的，但载脂蛋白A-I mRNA水平显着低于大鼠喂甜菜纤维比那些喂其他饮食。甜菜纤维饲料组大鼠的载脂蛋白A-IV mRNA有降低的趋势（P < 0.1）。这些数据表明，甜菜纤维的降胆固醇作用与肝脏载脂蛋白A-I基因表达减少有关。
Rats were fed a cholesterol-free diet with no added fiber (fiber-free) or with 15 g/100 g beet fiber or 5 g/100 g cholestyramine for 14 d. Final plasma total cholesterol concentrations were significantly lower in rats fed beet fiber than in those fed fiber-free or cholestyramine diets. This difference was due mainly to lower HDL cholesterol concentrations. The group fed beet fiber also tended (P < 0.1) to have lower apolipoprotein A-I concentration in plasma. Northern blot analysis revealed that the relative concentrations of jejunal apolipoprotein A-I and A-IV mRNA were the same in all groups, whereas ileal apolipoprotein A-I and A-IV mRNA levels were significantly lower in rats fed beet fiber or cholestyramine than in those fed the fiber-free diet. Hepatic apolipoprotein E mRNA concentrations were the same in all groups, but apolipoprotein A-I mRNA levels were significantly lower in rats fed beet fiber than in those fed the other diets. Apolipoprotein A-IV mRNA tended (P < 0.1) to be lower in rats fed the beet fiber diet. These data suggest that the hypocholesterolemic effect of dietary beet fiber is associated with diminished expression of the hepatic apolipoprotein A-I gene.