The glomerular basement membrane as a model system to study the bioactivity of heparan sulfate glycosaminoglycans.
The glomerular basement membrane as a model system to study the bioactivity of heparan sulfate glycosaminoglycans.
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DOI：
10.1017/s1431927611012682
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发表时间：
2012-02
影响因子：
2.8
通讯作者：
Wassenhove-McCarthy, Deborah J.
中科院分区：
文献类型：
--
作者：
McCarthy, Kevin J.;Wassenhove-McCarthy, Deborah J.
肾小球基底膜及其相关细胞是肾超滤过程中的关键因素。传统上认为，肾小球基底膜中与几个碳水化合物部分相关的阴离子电荷形成电荷选择性屏障，限制阴离子蛋白质通过肾小球基底膜进入尿腔。电荷选择功能，以及基底膜的大小选择成分，用于限制血浆蛋白从毛细血管管腔外流。硫酸乙酰肝素糖胺聚糖是附着在蛋白多糖核心蛋白上的带负电荷的碳水化合物结构，在建立肾小球基底膜的电荷选择功能中起作用。虽然文献中有大量的研究支持这一概念，但最近几项利用分子遗传学方法最小化肾小球基底膜阴离子电荷的研究结果表明，硫酸乙酰肝素在肾小球毛细血管壁中的作用仍未完全解决，这表明这一研究领域仍需新的和新的探索。
The glomerular basement membrane and its associated cells are critical elements in the renal ultrafiltration process. Traditionally the anionic charge associated with several carbohydrate moieties in the glomerular basement membrane are thought to form a charge selective barrier that restricts the transmembrane flux of anionic proteins across the glomerular basement membrane into the urinary space. The charge selective function, along with the size selective component of the basement membrane, serves to limit the efflux of plasma proteins from the capillary lumen. Heparan sulfate glycosaminoglycans are anionically charged carbohydrate structures attached to proteoglycan core proteins and have a role in establishing the charge selective function of the glomerular basement membrane. Although there are a large number of studies in the literature that support this concept, the results of several recent studies using molecular genetic approaches to minimize the anionic charge of the glomerular basement membrane would suggest that the role of heparan sulfate glycosaminoglycans in the glomerular capillary wall are still not yet entirely resolved, suggesting that this research area still requires new and novel exploration.