Comparison of Edwardsiella ictaluri isolates from different hosts and geographic origins
Comparison of Edwardsiella ictaluri isolates from different hosts and geographic origins
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DOI：
10.1111/jfd.12431
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发表时间：
2016-08-01
影响因子：
2.5
通讯作者：
Soto, E.
中科院分区：
文献类型：
--
作者：
Griffin, M. J.;Reichley, S. R.;Soto, E.
E.调查了来自不同来源的ictaluri分离物。从美国密西西比的养殖鲶鱼（斑点叉尾鱼）、西半球养殖的罗非鱼（尼罗罗非鱼）和美国佛罗里达繁殖的斑马鱼（斑马鱼）中回收分离株。这些分离株表型同源，抗菌谱很大程度上相似。从遗传学上讲，分离物具有差异，可以利用重复序列介导的PCR和gyrB序列，确定了三个不同的E。ictaluri基因型：一个与鲶鱼有关，一个来自罗非鱼，第三个来自斑马鱼。质粒图谱也具有组特异性，并与rep-PCR和gyrB序列相关。鲶鱼分离株具有E.然而，来自斑马鱼和罗非鱼分离物的质粒在组成和排列上都不同。此外，一些斑马鱼和罗非鱼分离物对几种E.蜱毒力因子分离血清学异质性，作为血清暴露于鲶鱼分离的癍点叉尾鱼罗非鱼和斑马鱼分离的抗体活性降低。这项工作确定了三个遗传上不同的E。除了抗菌和血清学分析外，还使用rep-PCR、16 S、gyrB和质粒测序对来自不同来源的ictaluri进行了鉴定。
The intraspecific variability of E. ictaluri isolates from different origins was investigated. Isolates were recovered from farm-raised catfish (Ictalurus punctatus) in Mississippi, USA, tilapia (Oreochromis niloticus) cultured in the Western Hemisphere and zebrafish (Danio rerio) propagated in Florida, USA. These isolates were phenotypically homologous and antimicrobial profiles were largely similar. Genetically, isolates possessed differences that could be exploited by repetitive-sequence-mediated PCR and gyrB sequence, which identified three distinct E. ictaluri genotypes: one associated with catfish, one from tilapia and a third from zebrafish. Plasmid profiles were also group specific and correlated with rep-PCR and gyrB sequences. The catfish isolates possessed profiles typical of those described for E. ictaluri isolates; however, plasmids from the zebrafish and tilapia isolates differed in both composition and arrangement. Furthermore, some zebrafish and tilapia isolates were PCR negative for several E. ictaluri virulence factors. Isolates were serologically heterogenous, as serum from a channel catfish exposed to a catfish isolate had reduced antibody activity to tilapia and zebrafish isolates. This work identifies three genetically distinct strains of E. ictaluri from different origins using rep-PCR, 16S, gyrB and plasmid sequencing, in addition to antimicrobial and serological profiling.